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Lactoperoxidase-med...
Lactoperoxidase-mediated degradation of single-walled carbon nanotubes in the presence of pulmonary surfactant
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- Bhattacharya, K. (författare)
- Karolinska Institutet
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- El-Sayed, R. (författare)
- Karolinska Institute, Sweden,Karolinska Inst, Inst Environm Med, Div Mol Toxicol, S-17177 Stockholm, Sweden
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- Andón, F. T. (författare)
- Karolinska Institute, Sweden,Karolinska Inst, Inst Environm Med, Div Mol Toxicol, S-17177 Stockholm, Sweden
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- Mukherjee, S. P. (författare)
- Karolinska Institute, Sweden,Karolinska Inst, Inst Environm Med, Div Mol Toxicol, S-17177 Stockholm, Sweden
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- Gregory, J. (författare)
- Karolinska Institute, Sweden,Karolinska Inst, Ctr Allergy Res, S-17177 Stockholm, Sweden;Karolinska Inst, Inst Environm Med, Div Physiol, S-17177 Stockholm, Sweden
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- Li, Hu (författare)
- Uppsala universitet,Tillämpad materialvetenskap
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- Zhao, Yinchen (författare)
- KTH,Funktionella material, FNM,KTH Royal Institute of Technology, Sweden,Royal Inst Technol, Dept Mat & Nanophys, Funct Mat Div, S-16440 Stockholm, Sweden
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- Seo, W. (författare)
- University of Pittsburgh, US,Univ Pittsburgh, Dept Chem, Pittsburgh, PA 15260 USA
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- Fornara, A. (författare)
- RISE,SP Kemi Material och Ytor,SP Tech Res Inst Sweden, Unit Chem Mat & Surfaces, S-11486 Stockholm, Sweden
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- Brandner, B. (författare)
- Karolinska Institutet,RISE,SP – Sveriges Tekniska Forskningsinstitut,SP Tech Res Inst Sweden, Unit Chem Mat & Surfaces, S-11486 Stockholm, Sweden
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- Toprak, Muhammet Sadaka (författare)
- KTH,Funktionella material, FNM,KTH Royal Institute of Technology, Sweden,Royal Inst Technol, Dept Mat & Nanophys, Funct Mat Div, S-16440 Stockholm, Sweden
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- Leifer, Klaus (författare)
- Uppsala universitet,Tillämpad materialvetenskap
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- Star, A. (författare)
- University of Pittsburgh, USA,Univ Pittsburgh, Dept Chem, Pittsburgh, PA 15260 USA
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- Fadeel, B. (författare)
- Karolinska Institute, Sweden,Karolinska Inst, Inst Environm Med, Div Mol Toxicol, S-17177 Stockholm, Sweden
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(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- Engelska.
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Ingår i: Carbon. - : Elsevier BV. - 0008-6223 .- 1873-3891. ; 91, s. 506-517
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Carbon nanotubes (CNTs) may elicit inflammatory responses following pulmonary exposure. Conversely, enzymatic biodegradation of CNTs by inflammatory cells has also been reported. The aim of this study was to study the degradation of oxidized single-walled CNTs (ox-SWCNTs) by lactoperoxidase (LPO), a secreted peroxidase present in the airways, and whether pulmonary surfactant affects this biodegradation. To this end, ox-SWCNTs were incubated in vitro with recombinant bovine LPO + H2O2 + NaSCN in the presence and absence of porcine lung surfactant (Curosurf®) and biodegradation was monitored using UV-Vis-NIR spectroscopy, Raman spectroscopy, and scanning electron microscopy. The interaction of recombinant LPO with bundles of ox-SWCNTs was confirmed by atomic force microscopy. Cell-free biodegradation of ox-SWCNTs was also observed ex vivo in murine bronchoalveolar lavage fluid in the presence of H2O2 + NaSCN. Our study provides evidence for biodegradation of ox-SWCNTs with a lung surfactant 'bio-corona' and expands the repertoire of mammalian peroxidases capable of biodegradation of ox-SWCNTs. These findings are relevant to inhalation exposure to these materials, as LPO serves as an important component of the airway defense system.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Annan teknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Other Engineering and Technologies (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Atomic force microscopy
- Biodegradation
- Biological organs
- Carbon
- Mammals
- Microbiology
- Near infrared spectroscopy
- Phospholipids
- Scanning electron microscopy
- Surface active agents
- Yarn
- Bronchoalveolar lavage fluid
- Inflammatory cells
- Inflammatory response
- Inhalation exposure
- Lactoperoxidase
- Lung surfactants
- Mammalian peroxidase
- Pulmonary surfactants
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
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Carbon
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- Av författaren/redakt...
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Bhattacharya, K.
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El-Sayed, R.
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Andón, F. T.
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Mukherjee, S. P.
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Gregory, J.
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Li, Hu
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visa fler...
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Zhao, Yinchen
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Seo, W.
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Fornara, A.
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Brandner, B.
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Toprak, Muhammet ...
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Leifer, Klaus
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Star, A.
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Fadeel, B.
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- Om ämnet
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Annan teknik
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
- Artiklar i publikationen
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Carbon
- Av lärosätet
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Kungliga Tekniska Högskolan
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RISE
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Uppsala universitet
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Karolinska Institutet