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Folding of insulin-...
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Hober, SophiaKTH,Biokemi och biokemisk teknologi
(författare)
Folding of insulin-like growth factor I is thermodynamically controlled by insulin-like growth factor binding protein.
- Artikel/kapitelEngelska1994
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2002-05-01
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American Chemical Society (ACS),1994
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LIBRIS-ID:oai:DiVA.org:kth-184213
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-184213URI
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https://doi.org/10.1021/bi00188a003DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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NR 20160331
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Insulin-like growth factor I (IGF-I) is thermodynamically unable to quantitatively form its native disulfides under reversible redox conditions in vitro [Hober et al. (1992) Biochemistry 31, 1749-1756]. These results prompted the question of how IGF-I may overcome this energetic problem in its folding in vivo. Here, we report that an IGF-I precursor, IGF-I-Ea, shows disulfide-exchange folding properties similar to those of mature IGF-I and, thus, is concluded not to overcome the identified folding problem of mature IGF-I. However, correct disulfide bonds are formed very efficiently when insulin-like growth factor binding protein 1 is added in equimolar amounts to IGF-I to the refolding mixture. On the basis of these results, we propose that one important function of at least one of the six homologous insulin-like growth factor binding proteins is to assist in the formation and maintenance of the native disulfides of IGF-I. To our knowledge, this is the first example where the folding of a mammalian protein or peptide in circulation has been demonstrated to be thermodynamically controlled by its binding protein. Speculatively, this could provide a mechanism to regulate the half-life of IGF-I in vivo by altering the interaction with insulin-like growth factor binding proteins.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Hansson, A
(författare)
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Uhlén, MathiasKTH,Biokemi och biokemisk teknologi(Swepub:kth)u1dulvmw
(författare)
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Nilsson, B
(författare)
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KTHBiokemi och biokemisk teknologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Biochemistry: American Chemical Society (ACS)33:220006-29601520-4995
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