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Tissue microarray p...
Tissue microarray profiling in human heart failure
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- Lal, S. (författare)
- Univ Sydney, Bosch Inst, Sydney Med Sch, Dept Anat & Histol, Sydney, NSW, Australia.
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- Nguyen, L. (författare)
- Univ Sydney, Bosch Inst, Sydney Med Sch, Dept Anat & Histol, Sydney, NSW, Australia.
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- Tezone, R. (författare)
- Univ Sydney, Bosch Inst, Sydney Med Sch, Dept Anat & Histol, Sydney, NSW, Australia.
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- Pontén, Fredrik (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,KTH, Sch Biotechnol, Royal Inst Technol, Dept Prote,Sci Life Lab, Stockholm, Sweden.
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- Odeberg, J. (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi
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- Li, A. (författare)
- Univ Sydney, Bosch Inst, Sydney Med Sch, Dept Anat & Histol, Sydney, NSW, Australia.
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- dos Remedios, C. (författare)
- Univ Sydney, Bosch Inst, Sydney Med Sch, Dept Anat & Histol, Sydney, NSW, Australia.
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Univ Sydney, Bosch Inst, Sydney Med Sch, Dept Anat & Histol, Sydney, NSW, Australia Proteomik och nanobioteknologi (creator_code:org_t)
- 2016-08-11
- 2016
- Engelska.
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Ingår i: Proteomics. - : Wiley-VCH Verlagsgesellschaft. - 1615-9853 .- 1615-9861.
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Tissue MicroArrays (TMAs) are a versatile tool for high-throughput protein screening, allowing qualitative analysis of a large number of samples on a single slide. We have developed a customizable TMA system that uniquely utilizes cryopreserved human cardiac samples from both heart failure and donor patients to produce formalin-fixed paraffin-embedded sections. Confirmatory upstream or downstream molecular studies can then be performed on the same (biobanked) cryopreserved tissue. In a pilot study, we applied our TMAs to screen for the expression of four-and-a-half LIM-domain 2 (FHL2), a member of the four-and-a-half LIM family. This protein has been implicated in the pathogenesis of heart failure in a variety of animal models. While FHL2 is abundant in the heart, not much is known about its expression in human heart failure. For this purpose, we generated an affinity-purified rabbit polyclonal anti-human FHL2 antibody. Our TMAs allowed high-throughput profiling of FHL2 protein using qualitative and semiquantitative immunohistochemistry that proved complementary to Western blot analysis. We demonstrated a significant relative reduction in FHL2 protein expression across different forms of human heart failure.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Cardiomyopathy
- Human heart failure
- Lim proteins
- Protein arrays
- Proteomic profiling
- Tissue microarray
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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