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Partial protection ...
Partial protection to respiratory syncytial virus (RSV) elicited in mice by intranasal immunization using live staphylococci with surface-displayed RSV-peptides
- Artikel/kapitelEngelska2000
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:kth-19795
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-19795URI
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https://doi.org/10.1016/S0264-410X(00)00063-3DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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QC 20100525
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A live bacterial vaccine-delivery system based on the food-grade bacterium Staphylococcus carnosus was used for delivery of peptides from the G glycoprotein of human respiratory syncytial virus, subtype A (RSV-A). Three peptides, corresponding to the G protein amino acids, 144-159 (denoted G5), 190-203 (G9) and 171-188 (G4 S), the latter with four cysteine residues substituted for serines, were expressed by recombinant means as surface-exposed on three different bacteria, and their surface accessibility on the bacteria was verified by fluorescence-activated cell sorting (FACS). Intranasal immunization of mice with the live recombinant staphylococci elicited significant anti-peptide as well as anti-virus serum IgG responses of balanced IgG1/IgG2a isotype profiles, and upon viral challenge with 10(5) tissue culture infectious doses(50) (TCID50), lung protection was demonstrated for approximately half of the mice in the G9 and G4 S immunization groups. To our knowledge, this is the first study in which protective immunity to a viral pathogen has been evoked using food-grade bacteria as vaccine-delivery vehicles.
Ämnesord och genrebeteckningar
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live bacterial vaccine delivery
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respiratory syncytial virus
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Staphylococcus carnosus
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recombinant lactococcus-lactis
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streptococcal protein-g
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albumin-binding region
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g fusion protein
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g-glycoprotein
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cell-wall
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klebsiella-pneumoniae
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antibody-responses
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oral vaccination
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balb/c mice
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Plotnicky-Gilquin, H.
(författare)
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Nguyen, T. N.
(författare)
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Liljeqvist, S.
(författare)
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Samuelson, PatrikKTH,Bioteknologi(Swepub:kth)u137srym
(författare)
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Bonnefoy, J. Y.
(författare)
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Ståhl, StefanKTH,Bioteknologi(Swepub:kth)u1svy8i4
(författare)
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Robert, A.
(författare)
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KTHBioteknologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Vaccine18:24, s. 2743-27520264-410X1873-2518
Internetlänk
Hitta via bibliotek
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Vaccine
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