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Sökning: onr:"swepub:oai:DiVA.org:kth-202835" > Evolutionary engine...

  • Fletcher, Eugene,1986Chalmers tekniska högskola,Chalmers University of Technology (författare)

Evolutionary engineering reveals divergent paths when yeast is adapted to different acidic environments

  • Artikel/kapitelEngelska2017

Förlag, utgivningsår, omfång ...

  • Academic Press,2017
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:kth-202835
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-202835URI
  • https://doi.org/10.1016/j.ymben.2016.10.010DOI
  • https://research.chalmers.se/publication/246664URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • QC 20170320
  • Tolerance of yeast to acid stress is important for many industrial processes including organic acid production. Therefore, elucidating the molecular basis of long term adaptation to acidic environments will be beneficial for engineering production strains to thrive under such harsh conditions. Previous studies using gene expression analysis have suggested that both organic and inorganic acids display similar responses during short term exposure to acidic conditions. However, biological mechanisms that will lead to long term adaptation of yeast to acidic conditions remains unknown and whether these mechanisms will be similar for tolerance to both organic and inorganic acids is yet to be explored. We therefore evolved Saccharomyces cerevisiae to acquire tolerance to HCl (inorganic acid) and to 0.3 M L-lactic acid (organic acid) at pH 2.8 and then isolated several low pH tolerant strains. Whole genome sequencing and RNA-seq analysis of the evolved strains revealed different sets of genome alterations suggesting a divergence in adaptation to these two acids. An altered sterol composition and impaired iron uptake contributed to HCl tolerance whereas the formation of a multicellular morphology and rapid lactate degradation was crucial for tolerance to high concentrations of lactic acid. Our findings highlight the contribution of both the selection pressure and nature of the acid as a driver for directing the evolutionary path towards tolerance to low pH. The choice of carbon source was also an important factor in the evolutionary process since cells evolved on two different carbon sources (raffinose and glucose) generated a different set of mutations in response to the presence of lactic acid. Therefore, different strategies are required for a rational design of low pH tolerant strains depending on the acid of interest.

Ämnesord och genrebeteckningar

  • NATURVETENSKAP Biologi hsv//swe
  • NATURAL SCIENCES Biological Sciences hsv//eng
  • Adaptive laboratory evolution
  • Lactic acid
  • Low pH
  • Yeast
  • Biology
  • Gene expression
  • Genes
  • Inorganic acids
  • Organic acids
  • Plants (botany)
  • Biological mechanisms
  • Different carbon sources
  • Evolutionary engineering
  • Evolutionary process
  • Gene expression analysis
  • Organic acid productions
  • Whole genome sequencing

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Feizi, Amir,1980Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)feizi (författare)
  • Bisschops, Mark,1985Chalmers tekniska högskola,Chalmers University of Technology,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)(Swepub:cth)bishops (författare)
  • Hallström, Björn M.KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1ih19oh (författare)
  • Khoomrung, Sakda,1978Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)sakda (författare)
  • Siewers, Verena,1976Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)siewers (författare)
  • Nielsen, JensKTH,Genteknologi,Science for Life Laboratory, SciLifeLab,Chalmers University of Technology, Sweden,Chalmers tekniska högskola(Swepub:cth)nielsenj (författare)
  • Chalmers tekniska högskolaKungliga Tekniska Högskolan (KTH) (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Metabolic engineering: Academic Press39, s. 19-281096-71761096-7184

Internetlänk

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