Sökning: onr:"swepub:oai:DiVA.org:kth-20389" >
Characterisation of...
-
Jorgensen, P. M.
(författare)
Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex
- Artikel/kapitelEngelska2001
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:kth-20389
-
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-20389URI
-
https://doi.org/10.1016/S0378-1119(00)00511-4DOI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:1936715URI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
QC 20100525
-
Accurate segregation of sister chromatids during mitosis is necessary to avoid the aneuploidy found in many cancers. The spindle checkpoint, which monitors the metaphase to anaphase transition, has been shown to be defective in cancers with chromosomal instability. This checkpoint regulates the anaphase-promoting complex or cyclosome (APC/C), a cell cycle ubiquitin ligase regulating among other things sister chromatid separation. We have previously investigated the mouse Apc1 protein (previously also called Tsg24), the largest subunit of the APC/C. We have now sequenced a full-length human APC1 cDNA, mapped its chromosomal location, and analysed its intron-exon boundaries. We have also investigated the RNA and protein expression of the Apc1 and other APC/C components in normal and cancer cells and the relative occurrence of expressed sequence tugs (ESTs) representing APC subunits from different tissues. The different APC/C subunits are expressed in most tissues and cell types at fairly constant levels relative to each other, suggesting that they perform their functions as part of a complex. A difference from this pattern is however seen for the APC6, which in some cases is more strongly expressed, suggesting a special function for this protein in certain tissues and cell types.
Ämnesord och genrebeteckningar
-
cell cycle
-
mitosis
-
ubiquitination
-
chromosome segregation
-
metaphase
-
scf ubiquitin-ligase
-
mammalian-cells
-
human cancers
-
protein
-
mitosis
-
division
-
spindle
-
form
-
rbx1
-
dna
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Graslund, S.
(författare)
-
Betz, R.
(författare)
-
Ståhl, StefanKTH,Bioteknologi(Swepub:kth)u1svy8i4
(författare)
-
Larsson, C.Karolinska Institutet
(författare)
-
Hoog, C.Karolinska Institutet
(författare)
-
KTHBioteknologi
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Gene262:02-jan, s. 51-590378-11191879-0038
Internetlänk
Hitta via bibliotek
-
Gene
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas