Sökning: onr:"swepub:oai:DiVA.org:kth-205503" > The beta-1,3-glucan...
Fältnamn | Indikatorer | Metadata |
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000 | 03288naa a2200409 4500 | |
001 | oai:DiVA.org:kth-205503 | |
003 | SwePub | |
008 | 170510s2017 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2055032 URI |
024 | 7 | a https://doi.org/10.1111/cmi.126592 DOI |
040 | a (SwePub)kth | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Samalova, Marketa4 aut |
245 | 1 0 | a The beta-1,3-glucanosyltransferases (Gels) affect the structure of the rice blast fungal cell wall during appressorium-mediated plant infection |
264 | c 2016-10-11 | |
264 | 1 | b Wiley-VCH Verlagsgesellschaft,c 2017 |
338 | a print2 rdacarrier | |
500 | a QC 20170510 | |
520 | a The fungal wall is pivotal for cell shape and function, and in interfacial protection during host infection and environmental challenge. Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae. We focus on the family of glucan elongation proteins (Gels) and characterize five putative beta-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature. We generated targeted deletion mutants of all Gel isoforms, that is, the GH72(+), which carry a putative carbohydrate-binding module, and the GH72(-)Gels, without this motif. We reveal that M. oryzae GH72(+) GELs are expressed in spores and during both infective and vegetative growth, but each individual Gel enzymes are dispensable for pathogenicity. Further, we demonstrated that Delta gel1 Delta gel3 Delta gel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain. The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues). We conclude that Gel proteins play significant roles in structural modification of the fungal cell wall during appressorium-mediated plant infection. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Medicinsk bioteknologi0 (SwePub)304012 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Medical Biotechnology0 (SwePub)304012 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
700 | 1 | a Melida, Hugou KTH,Glykovetenskap4 aut0 (Swepub:kth)u140zccu |
700 | 1 | a Vilaplana, Francisco,d 1979-u KTH,Glykovetenskap4 aut0 (Swepub:kth)u173a4yy |
700 | 1 | a Bulone, Vincentu KTH,Glykovetenskap4 aut0 (Swepub:kth)u13o1auq |
700 | 1 | a Soanes, Darren M.4 aut |
700 | 1 | a Talbot, Nicholas J.4 aut |
700 | 1 | a Gurr, Sarah J.4 aut |
710 | 2 | a KTHb Glykovetenskap4 org |
773 | 0 | t Cellular Microbiologyd : Wiley-VCH Verlagsgesellschaftg 19:3q 19:3x 1462-5814x 1462-5822 |
856 | 4 | u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/cmi.12659 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-205503 |
856 | 4 8 | u https://doi.org/10.1111/cmi.12659 |
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