Sökning: onr:"swepub:oai:DiVA.org:kth-208425" >
The terpene synthas...
-
Hansen, N. L.
(författare)
The terpene synthase gene family in Tripterygium wilfordii harbors a labdane-type diterpene synthase among the monoterpene synthase TPS-b subfamily
- Artikel/kapitelEngelska2017
Förlag, utgivningsår, omfång ...
-
2017-02-14
-
Blackwell Publishing,2017
-
printrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:kth-208425
-
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-208425URI
-
https://doi.org/10.1111/tpj.13410DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
QC 20170609
-
Tripterygium wilfordii (Celastraceae) is a medicinal plant with anti-inflammatory and immunosuppressive properties. Identification of a vast array of unusual sesquiterpenoids, diterpenoids and triterpenoids in T. wilfordii has spurred investigations of their pharmacological properties. The tri-epoxide lactone triptolide was the first of many diterpenoids identified, attracting interest due to the spectrum of bioactivities. To probe the genetic underpinning of diterpenoid diversity, an expansion of the class II diterpene synthase (diTPS) family was recently identified in a leaf transcriptome. Following detection of triptolide and simple diterpene scaffolds in the root, we sequenced and mined the root transcriptome. This allowed identification of the root-specific complement of TPSs and an expansion in the class I diTPS family. Functional characterization of the class II diTPSs established their activities in the formation of four C-20 diphosphate intermediates, precursors of both generalized and specialized metabolism and a novel scaffold for Celastraceae. Functional pairs of the class I and II enzymes resulted in formation of three scaffolds, accounting for some of the terpenoid diversity found in T. wilfordii. The absence of activity-forming abietane-type diterpenes encouraged further testing of TPSs outside the canonical class I diTPS family. TwTPS27, close relative of mono-TPSs, was found to couple with TwTPS9, converting normal-copalyl diphosphate to miltiradiene. The phylogenetic distance to established diTPSs indicates neo-functionalization of TwTPS27 into a diTPS, a function not previously observed in the TPS-b subfamily. This example of evolutionary convergence expands the functionality of TPSs in the TPS-b family and may contribute miltiradiene to the diterpenoids of T. wilfordii.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Heskes, A. M.
(författare)
-
Hamberger, B.
(författare)
-
Olsen, C. E.
(författare)
-
Hallström, Björn M.KTH,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1ih19oh
(författare)
-
Andersen-Ranberg, J.
(författare)
-
KTHScience for Life Laboratory, SciLifeLab
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:The Plant Journal: Blackwell Publishing89:3, s. 429-4410960-74121365-313X
Internetlänk
Hitta via bibliotek
Till lärosätets databas