Sökning: onr:"swepub:oai:DiVA.org:kth-20886" > Microparticles for ...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 02217naa a2200421 4500 | |
001 | oai:DiVA.org:kth-20886 | |
003 | SwePub | |
008 | 100810s2001 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-208862 URI |
024 | 7 | a https://doi.org/10.1002/1522-2683(200107)22:12<2384::AID-ELPS2384>3.0.CO;2-W2 DOI |
040 | a (SwePub)kth | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Nilsson, H.4 aut |
245 | 1 0 | a Microparticles for selective protein determination in capillary electrophoresis |
264 | 1 | c 2001 |
338 | a print2 rdacarrier | |
500 | a QC 20100525 | |
520 | a A system for detection of trace amounts of protein was developed. Two different monoclonal antibodies against human chorionic gonadotropin (hCG) were covalently bound to latex particles. When the latex particles were mixed with a sample containing hCG, a latex-protein-latex complex (immunocomplex) was formed. The complex was separated from the single latex particles using capillary electrophoresis and detected using UV-Vis detection. Limit of detection was 8 amol hCG. The separation was also monitored in real time using laser induced fluorescence - charge coupled device (LIF-CCD) imaging detection. However, a limitation of the method is the restriction to detection of proteins for which monoclonal antibodies are available. | |
653 | a capillary electrophoresis | |
653 | a particles | |
653 | a immunoassay | |
653 | a imaging | |
653 | a acoustic trapping | |
653 | a layer immunoaffinity chromatography | |
653 | a fluorescence | |
653 | a separations | |
653 | a quantitation | |
700 | 1 | a Wiklund, Martinu KTH,Fysik4 aut0 (Swepub:kth)u1rillco |
700 | 1 | a Johansson, T.4 aut |
700 | 1 | a Hertz, Hans M.u KTH,Fysik4 aut0 (Swepub:kth)u10uiefj |
700 | 1 | a Nilsson, S.4 aut |
710 | 2 | a KTHb Fysik4 org |
773 | 0 | t Electrophoresisg 22:12, s. 2384-2390q 22:12<2384-2390x 0173-0835x 1522-2683 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-20886 |
856 | 4 8 | u https://doi.org/10.1002/1522-2683(200107)22:12<2384::AID-ELPS2384>3.0.CO;2-W |
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