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Regulation of subun...
Regulation of subunit-specific germinal center B cell responses to the HIV-1 envelope glycoproteins by antibody-mediated feedback
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- Forsell, Mattias N. E. (författare)
- Umeå universitet,Immunologi/immunkemi,Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
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- Kvastad, Linda (författare)
- KTH,Genteknologi
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- Sedimbi, S. K. (författare)
- Karolinska Institutet
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- Andersson, J. (författare)
- Karolinska Institutet
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- Karlsson, M. C. I. (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2017-06-30
- 2017
- Engelska.
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Ingår i: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 8:JUN
- Relaterad länk:
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https://doi.org/10.3...
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https://www.frontier...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- The regulation of germinal center (GC) B cell responses to single epitopes is well investigated. How monoclonal B cells are regulated within the polyclonal B cell response to protein antigens is less so. Here, we investigate the primary GC B cell response after injection of mice with HIV-1 envelope glycoproteins. We demonstrate that single GCs are seeded by a diverse number of B cell clones shortly after a single immunization and that the presence of Env-specific antibodies can inhibit the development of early GC B cells. Importantly, the suppression was dependent on the GC B cells and the infused antibodies to target the same subunit of the injected HIV-1 envelope glycoproteins. An affinity-dependent antibody feedback has previously been shown to regulate GC B cell development. Here, we propose that this antibody-based feedback acts on GC B cells only if they target the same or overlapping epitopes. This study provides important basic information of GC B cell regulation, and for future vaccine designs with aim to elicit neutralizing antibodies against HIV-1.
Ämnesord
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Envelope glycoproteins
- Epitope-specific antibodies
- Epitope-specific B cells
- HIV-1
- Regulation of germinal centers
- virus glycoprotein
- affinity chromatography
- animal tissue
- antibody mediated rejection
- Article
- biotinylation
- cell selection
- cellular immunity
- clonogenesis
- controlled study
- enzyme linked immunosorbent assay
- feedback system
- flow cytometry
- genome size
- germinal center
- Human immunodeficiency virus 1
- immunization
- immunofluorescence microscopy
- immunohistochemistry
- laser microdissection
- mouse
- nonhuman
- polymerase chain reaction
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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