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High-density serum/...
High-density serum/plasma reverse phase protein arrays
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- Hellström, Cecilia (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Affinity Proteomics
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- Dodig-Crnković, Tea (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Affinity Proteomics
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- Hong, Mun-Gwan (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Affinity Proteomics
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- Schwenk, Jochen (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Affinity Proteomics
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- Nilsson, Peter (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Affinity Proteomics
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- Sjöberg, Ronald (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Affinity Proteomics
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(creator_code:org_t)
- 2017-07-04
- 2017
- Engelska.
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Ingår i: Serum/Plasma Proteomics. - New York, NY : Humana Press. ; , s. 229-238
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In-depth exploration and characterization of human serum and plasma proteomes is an attractive strategy for the identification of potential prognostic or diagnostic biomarkers. The possibility of analyzing larger numbers of samples in a high-throughput fashion has markedly increased with affinity-based microarrays, thus providing higher statistical power to these biomarker studies. Here, we describe a protocol for high-density serum and plasma reverse phase protein arrays (RPPAs). We demonstrate how a biobank of 12,392 samples was immobilized and analyzed on a single microarray slide, allowing high-quality profiling of abundant target proteins across all samples in one assay.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Affinity proteomics
- Fluorescent detection
- Noncontact inkjet printer
- Plasma
- Protein profiling
- Reverse phase protein array
- RPPA
- Serum
Publikations- och innehållstyp
- ref (ämneskategori)
- kap (ämneskategori)
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