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Synthesis and bindi...
Synthesis and binding affinity analysis of alpha 1-2-and alpha 1-6-O/S-linked dimannosides for the elucidation of sulfur in glycosidic bonds using quartz crystal microbalance sensors
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- Norberg, Oscar (författare)
- KTH,Kemi
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Wu, Bin (författare)
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- Thota, Niranjan (författare)
- KTH,Kemi
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Ge, Jian-Tao (författare)
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- Fauquet, Germain (författare)
- KTH,Kemi
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Saur, Ann-Kathrin (författare)
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Aastrup, Teodor (författare)
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Dong, Hai (författare)
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- Yan, Mingdi (författare)
- KTH,Kemi,Department of Chemistry, University of Massachusetts Lowell, 1 University Ave., MA, 01854 Lowell, USA
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- Ramström, Olof (författare)
- KTH,Kemi,Department of Chemistry, University of Massachusetts Lowell, 1 University Ave., MA, 01854 Lowell, USA
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(creator_code:org_t)
- ELSEVIER SCI LTD, 2017
- 2017
- Engelska.
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Ingår i: Carbohydrate Research. - : ELSEVIER SCI LTD. - 0008-6215 .- 1873-426X. ; 452, s. 35-42
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The role of sulfur in glycosidic bonds has been evaluated using quartz crystal microbalance methodology. Synthetic routes towards alpha 1-2- and alpha 1-6-linked dimannosides with S-or O-glycosidic bonds have been developed, and the recognition properties assessed in competition binding assays with the cognate lectin concanavalin A. Mannose-presenting QCM sensors were produced using photoinitiated, nitrenemediated immobilization methods, and the subsequent binding study was performed in an automated flow-through instrumentation, and correlated with data from isothermal titration calorimetry. The recorded Kd-values corresponded well with reported binding affinities for the O-linked dimannosides with affinities for the alpha 1-2-linked dimannosides in the lower micromolar range. The S-linked analogs showed slightly disparate effects, where the alpha 1-6-linked analog showed weaker affinity than the O-linked dimannoside, as well as positive apparent cooperativity, whereas the alpha 1-2-analog displayed very similar binding compared to the O-linked structure.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Photochemistry
- Thiosaccharides
- CuAAC
- Carbohydrates
- Lectins
- Biosensor
- Quartz crystal microbalance
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Till lärosätets databas
- Av författaren/redakt...
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Norberg, Oscar
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Wu, Bin
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Thota, Niranjan
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Ge, Jian-Tao
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Fauquet, Germain
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Saur, Ann-Kathri ...
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visa fler...
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Aastrup, Teodor
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Dong, Hai
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Yan, Mingdi
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Ramström, Olof
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
- Artiklar i publikationen
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Carbohydrate Res ...
- Av lärosätet
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Kungliga Tekniska Högskolan