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Covalently immobili...
Covalently immobilized Trp60Cys mutant of omega-transaminase from Chromobacterium violaceum for kinetic resolution of racemic amines in batch and continuous-flow modes
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Abahazi, Emese (författare)
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Satorhelyi, Peter (författare)
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Erdelyi, Balazs (författare)
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Vertessy, Beata G. (författare)
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- Land, Henrik (författare)
- KTH,Industriell bioteknologi,AlbaNova University Center
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Paizs, Csaba (författare)
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- Berglund, Per (författare)
- KTH,Industriell bioteknologi
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Poppe, Laszlo (författare)
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(creator_code:org_t)
- Elsevier, 2018
- 2018
- Engelska.
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Ingår i: Biochemical engineering journal. - : Elsevier. - 1369-703X .- 1873-295X. ; 132, s. 270-278
- Relaterad länk:
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http://www.sciencedi...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Covalent immobilization of an engineered omega-transaminase mutant Trp60Cys from Chromobacterium violaceum (CvTAW60C) was performed on bisepoxide-activated aminoalkyl resins. Activity of the various CvTAW60C preparations was evaluated in kinetic resolution of four racemic amines (rac-1aâd). The most active EA-G-CvTAW60C preparation (CvTAW60C attached to polymeric resin with ethylamine function activated with glycerol diglycidyl etherâEA-G) could perform the kinetic resolution of racemic 4-phenylbutan-2-amine (rac-1a) over 49% conversion up to 19 consecutive reaction cycles or in media containing up to 50% v/v DMSO as cosolvent in batch mode reactions. The immobilization process of CvTAW60C onto the EA-G resin filled in stainless steel bioreactors was also tested in flow-through mode. Kinetic resolution of three racemic amines containing aromatic moieties (rac-1a-c) was performed in continuous-flow mode resulting in easy-to-separate mixture of the corresponding ketone (2aâc) and the non-converted (R)-amine in high enantiopurity (ee(R)-1a-câ¯â¥â¯96%).
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Nyckelord
- Transaminase
- Covalent immobilization
- Amine
- Kinetic resolution
- Continuous-flow biocatalysis
- Bioteknologi
- Biotechnology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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