Sökning: onr:"swepub:oai:DiVA.org:kth-234561" >
Chemical characteri...
Chemical characterization and wound healing property of a beta-D-glucan from edible mushroom Piptoporus betulinus
-
- de Jesus, Liana Inara (författare)
- Univ Fed Parana, Dept Biochem & Mol Biol, CP 19046, Curitiba, PR, Brazil.
-
- Smiderle, Fhernanda R. (författare)
- Univ Fed Parana, Dept Biochem & Mol Biol, CP 19046, Curitiba, PR, Brazil.
-
- Ruthes, Andrea C. (författare)
- KTH,Glykovetenskap
-
visa fler...
-
- Vilaplana, Francisco, 1979- (författare)
- KTH,Fiber- och polymerteknologi
-
- Dal'Lin, Fernando Tonholi (författare)
- Univ Fed Parana, Dept Pharmacol, CP 19046, Curitiba, PR, Brazil.
-
- Maria-Ferreira, Daniele (författare)
- Univ Fed Parana, Dept Biochem & Mol Biol, CP 19046, Curitiba, PR, Brazil.;Univ Fed Parana, Dept Pharmacol, CP 19046, Curitiba, PR, Brazil.
-
- Werner, Maria Fernanda (författare)
- Univ Fed Parana, Dept Pharmacol, CP 19046, Curitiba, PR, Brazil.
-
- Van Griensven, Leo J. L. D. (författare)
- Wageningen Univ & Res, Plant Res Int, Bomsesteeg 1, NL-6708 PD Wageningen, Netherlands.
-
- Iacomini, Marcello (författare)
- Univ Fed Parana, Dept Biochem & Mol Biol, CP 19046, Curitiba, PR, Brazil.
-
visa färre...
-
Univ Fed Parana, Dept Biochem & Mol Biol, CP 19046, Curitiba, PR, Brazil Glykovetenskap (creator_code:org_t)
- Elsevier, 2018
- 2018
- Engelska.
-
Ingår i: International Journal of Biological Macromolecules. - : Elsevier. - 0141-8130 .- 1879-0003. ; 117, s. 1361-1366
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- A water-soluble beta-D-glucan was obtained from fruiting bodies of Piptoporus betulinus, by hot aqueous extraction followed by freeze-thawing procedure and dialysis. Its molar mass distribution and conformational behavior in solution was assessed by size-exclusion chromatography coupled with multiangle laser light scattering, showing a polysaccharide with an average molecular weight of 2.5 x 10(5) Da with a random coil conformation for molecular weights below 1 x 10(6) Da. Typical signals of beta-(1 -> 3)-linkages were observed in NMR spectrum (delta 102.7/4.76; 102.8/4.74; 102.9/4.52; and delta 85.1/3.78; 85.0/3.77) and also signals of O-6 substitution at delta 69.2/4.22 and 69.2/3.87. The analysis of partially O-methylated alditol acetates corroborates the NMR results, indicating the presence of a beta-D-glucan with a main chain (1 -> 3)-linked, substituted at O-6 by single-units of glucose. The beta-D-glucan showed no toxicity on human colon carcinoma cell line (Caco-2) up to 1000 mu g mL(-1) and promoted cell migration on in vitro scratch assay, demonstrating a potential wound healing capacity.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- beta-D-glucan
- Random coil
- Wound healing
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas