Galacturonate Metabolism in Anaerobic Chemostat Enrichment Cultures: Combined Fermentation and Acetogenesis by the Dominant sp nov "Candidatus Galacturonibacter soehngenii"

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Galacturonate Metabolism in Anaerobic Chemostat Enrichment Cultures : Combined Fermentation and Acetogenesis by the Dominant sp nov "Candidatus Galacturonibacter soehngenii"

Valk, Laura C. (författare): Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.

Frank, Jeroen (författare): Radboud Univ Nijmegen, Soehngen Inst Anaerob Microbiol, Nijmegen, Netherlands.

de la Torre-Cortes, Pilar (författare): Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.

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van 't Hof, Max (författare): Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.

van Maris, Antonius J. A., Professor, 1976- (författare): KTH,Industriell bioteknologi,Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.

Pronk, Jack T. (författare): Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.

van Loosdrecht, Mark C. M. (författare): Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.

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Delft Univ Technol, Dept Biotechnol, Delft, Netherlands Radboud Univ Nijmegen, Soehngen Inst Anaerob Microbiol, Nijmegen, Netherlands. (creator_code:org_t)

American Society for Microbiology, 2018
2018
Engelska.
Ingår i: Applied and Environmental Microbiology. - : American Society for Microbiology. - 0099-2240 .- 1098-5336. ; 84:18

Relaterad länk:: https://urn.kb.se/re...; visa fler...; https://doi.org/10.1...; visa färre...

Tidskriftsartikel (refereegranskat)

Abstract Ämnesord

Stäng

Agricultural residues such as sugar beet pulp and citrus peel are rich in pectin, which contains galacturonic acid as a main monomer. Pectin-rich residues are underexploited as feedstocks for production of bulk chemicals or biofuels. The anaerobic, fermentative conversion of D-galacturonate in anaerobic chemostat enrichment cultures provides valuable information toward valorization of these pectin-rich feedstocks. Replicate anaerobic chemostat enrichments, with D-galacturonate as the sole limiting carbon source and inoculum from cow rumen content and rotting orange peels, yielded stable microbial communities, which were dominated by a novel Lachnospiraceae species, for which the name "Candidatus Galacturonibacter soehngenii" was proposed. Acetate was the dominant catabolic product, with formate and H-2 as coproducts. The observed molar ratio of acetate and the combined amounts of H-2 and formate deviated significantly from 1, which suggested that some of the hydrogen and CO2 formed during D-galacturonate fermentation was converted into acetate via the Wood-Ljungdahl acetogenesis pathway. Indeed, metagenomic analysis of the enrichment cultures indicated that the genome of "Candidatus G. soehngenii" encoded enzymes of the adapted Entner-Doudoroff pathway for D-galacturonate metabolism as well as enzymes of the Wood-Ljungdahl pathway. The simultaneous operation of these pathways may provide a selective advantage under D-galacturonate-limited conditions by enabling a higher specific ATP production rate and lower residual D-galacturonate concentration than would be possible with a strictly fermentative metabolism of this carbon and energy source. IMPORTANCE This study on D-galacturonate metabolism by open, mixed-culture enrichments under anaerobic, D-galacturonate-limited chemostat conditions shows a stable and efficient fermentation of D-galacturonate into acetate as the dominant organic fermentation product. This fermentation stoichiometry and population analyses provide a valuable baseline for interpretation of the conversion of pectin-rich agricultural feedstocks by mixed microbial cultures. Moreover, the results of this study provide a reference for studies on the microbial metabolism of D-galacturonate under different cultivation regimes.

Ämnesord

NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)

Nyckelord

acetogenesis
enrichment culture
fermentation
galacturonate
metagenomics

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Av författaren/redakt...: Valk, Laura C.; Frank, Jeroen; de la Torre-Cort ...; van 't Hof, Max; van Maris, Anton ...; Pronk, Jack T.; visa fler...; van Loosdrecht, ...; visa färre...

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