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Efficacy of copolym...
Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration
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- Munir, Arooj (author)
- Univ Bergen, Ctr Clin Dent Res, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway.
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- Doskeland, Anne (author)
- Univ Bergen, Dept Biomed, Bergen, Norway.
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- Avery, Steven J. (author)
- Cardiff Univ, Dept Oral & Biomed Sci, Sch Dent, Cardiff, S Glam, Wales.;CITER, Cardiff, S Glam, Wales.
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- Fuoco, Tiziana, PhD, 1986- (author)
- KTH,Fiber- och polymerteknologi
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- Mohamed-Ahmed, Samih (author)
- Univ Bergen, Ctr Clin Dent Res, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway.
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- Lygre, Henning (author)
- Univ Bergen, Ctr Clin Dent Res, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway.
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- Finne Wistrand, Anna, 1976- (author)
- KTH,Fiber- och polymerteknologi,sDept Fibre & Polymer Technol, Stockholm, Sweden.
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- Sloan, Alastair J. (author)
- Cardiff Univ, Dept Oral & Biomed Sci, Sch Dent, Cardiff, S Glam, Wales.;CITER, Cardiff, S Glam, Wales.
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- Waddington, Rachel J. (author)
- Cardiff Univ, Dept Oral & Biomed Sci, Sch Dent, Cardiff, S Glam, Wales.;CITER, Cardiff, S Glam, Wales.
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- Mustafa, Kamal (author)
- Univ Bergen, Ctr Clin Dent Res, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway.
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- Suliman, Salwa (author)
- Univ Bergen, Ctr Clin Dent Res, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway.
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Univ Bergen, Ctr Clin Dent Res, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway Univ Bergen, Dept Biomed, Bergen, Norway. (creator_code:org_t)
- 2019-05-31
- 2019
- English.
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In: Journal of Tissue Engineering. - : SAGE PUBLICATIONS INC. - 2041-7314. ; 10
- Related links:
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https://doi.org/10.1...
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Abstract
Subject headings
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- Poly(L-lactide-co-epsilon-caprolactone) scaffolds were functionalised by 10 or 20 mu g/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 mu g/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7 days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21 days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21 days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Growth factor
- mesenchymal stem cell
- bone tissue engineering
- drug delivery
- functionalisation
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Munir, Arooj
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Doskeland, Anne
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Avery, Steven J.
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Fuoco, Tiziana, ...
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Mohamed-Ahmed, S ...
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Lygre, Henning
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show more...
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Finne Wistrand, ...
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Sloan, Alastair ...
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Waddington, Rach ...
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Mustafa, Kamal
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Suliman, Salwa
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Journal of Tissu ...
- By the university
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Royal Institute of Technology