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Label-Free Surface ...
Label-Free Surface Protein Profiling of Extracellular Vesicles by an Electrokinetic Sensor
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- Cavallaro, Sara (författare)
- KTH,Fotonik,KTH Royal Inst Technol, Sch Engn Sci, Dept Appl Phys, S-16440 Kista, Sweden
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- Horak, Josef (författare)
- KTH,Proteinvetenskap,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Prot Sci, AlbalNova Univ Ctr, S-10691 Stockholm, Sweden
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- Haag, Petra (författare)
- Karolinska Institutet
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- Gupta, Dhanu (författare)
- Karolinska Institutet
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- Stiller, Christiane (författare)
- KTH,Proteinvetenskap,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Prot Sci, AlbalNova Univ Ctr, S-10691 Stockholm, Sweden
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- Sahu, Siddharth S. (författare)
- Karolinska Institutet,Uppsala universitet,Fasta tillståndets elektronik
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- Gorgens, Andre (författare)
- Karolinska Institutet
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- Gatty, Hithesh K. (författare)
- Uppsala universitet,Fasta tillståndets elektronik
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- Viktorsson, Kristina (författare)
- Karolinska Institutet
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- El Andaloussi, Samir (författare)
- Karolinska Inst, Clin Res Ctr, Dept Lab Med, S-17177 Stockholm, Sweden.;Evox Therapeut Ltd, Oxford OX4 4HG, England.
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- Lewensohn, Rolf (författare)
- Karolinska Institutet
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- Eriksson Karlström, Amelie (författare)
- KTH,Proteinvetenskap,Sch Engn Sci Chem Biotechnol & Hlth, Dept Prot Sci, AlbalNova Univ Ctr, S-10691 Stockholm, Sweden.,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Prot Sci, AlbalNova Univ Ctr, S-10691 Stockholm, Sweden
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- Linnros, Jan, 1953- (författare)
- KTH,Fotonik,Sch Engn Sci, Dept Appl Phys, S-16440 Kista, Sweden.,KTH Royal Inst Technol, Sch Engn Sci, Dept Appl Phys, S-16440 Kista, Sweden
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- Dev, Apurba (författare)
- Uppsala universitet,Fasta tillståndets elektronik
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(creator_code:org_t)
- 2019-04-25
- 2019
- Engelska.
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Ingår i: ACS Sensors. - : AMER CHEMICAL SOC. - 2379-3694. ; 4:5, s. 1399-1408
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Small extracellular vesicles (sEVs) generated from the endolysosomal system, often referred to as exosomes, have attracted interest as a suitable biomarker for cancer diagnostics, as they carry valuable biological information and reflect their cells of origin. Herein, we propose a simple and inexpensive electrical method for label-free detection and profiling of sEVs in the size range of exosomes. The detection method is based on the electrokinetic principle, where the change in the streaming current is monitored as the surface markers of the sEVs interact with the affinity reagents immobilized on the inner surface of a silica microcapillary. As a proof-of-concept, we detected sEVs derived from the non-small-cell lung cancer (NSCLC) cell line H1975 for a set of representative surface markers, such as epidermal growth factor receptor (EGFR), CD9, and CD63. The detection sensitivity was estimated to be similar to 175000 sEVs, which represents a sensor surface coverage of only 0.04%. We further validated the ability of the sensor to measure the expression level of a membrane protein by using sEVs displaying artificially altered expressions of EGFR and CD63, which were derived from NSCLC and human embryonic kidney (HEK) 293T cells, respectively. The analysis revealed that the changes in EGFR and CD63 expressions in sEVs can be detected with a sensitivity in the order of 10% and 3%, respectively, of their parental cell expressions. The method can be easily parallelized and combined with existing microfluidic-based EV isolation technologies, allowing for rapid detection and monitoring of sEVs for cancer diagnosis.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- extracellular vesicles
- electrokinetic effect
- biosensor
- label-free
- protein profiling
- cancer
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Cavallaro, Sara
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Horak, Josef
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Haag, Petra
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Gupta, Dhanu
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Stiller, Christi ...
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Sahu, Siddharth ...
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visa fler...
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Gorgens, Andre
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Gatty, Hithesh K ...
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Viktorsson, Kris ...
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El Andaloussi, S ...
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Lewensohn, Rolf
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Eriksson Karlstr ...
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Linnros, Jan, 19 ...
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Dev, Apurba
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
- Artiklar i publikationen
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ACS Sensors
- Av lärosätet
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Kungliga Tekniska Högskolan
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Uppsala universitet
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Karolinska Institutet