Sökning: onr:"swepub:oai:DiVA.org:kth-255741" >
Systematic Developm...
Systematic Development of Sandwich Immunoassays for the Plasma Secretome
-
- Häussler, Ragna S. (författare)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Bendes, Annika (författare)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Iglesias, Maria Jesus (författare)
- KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Division of Internal Medicine, University Hospital of North Norway, Tromsø, 9010, Norway
-
visa fler...
-
- Sanchez-Rivera, Laura (författare)
- KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab
-
- Dodig-Crnkovic, Tea (författare)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Byström, Sanna (författare)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Fredolini, Claudia (författare)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Birgersson, Elin (författare)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Dale, Matilda (författare)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Edfors, Fredrik (författare)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab
-
- Fagerberg, Linn (författare)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab
-
- Rockberg, Johan (författare)
- KTH,Proteinteknologi
-
- Tegel, Hanna (författare)
- KTH,Proteinteknologi
-
- Uhlèn, Mathias (författare)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab,Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, 2970, Denmark
-
- Qundos, Ulrika (författare)
- Atlas Antibodies AB, Bromma, 168 69, Sweden
-
- Schwenk, Jochen M. (författare)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
visa färre...
-
(creator_code:org_t)
- 2019-07-22
- 2019
- Engelska.
-
Ingår i: Proteomics. - : Wiley. - 1615-9853 .- 1615-9861.
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://onlinelibrar...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The plasma proteome offers a clinically useful window into human health. Recent advances from highly multiplexed assays now call for appropriate pipelines to validate individual candidates. Here, a workflow is developed to build dual binder sandwich immunoassays (SIA) and for proteins predicted to be secreted into plasma. Utilizing suspension bead arrays, ≈1800 unique antibody pairs are first screened against 209 proteins with recombinant proteins as well as EDTA plasma. Employing 624 unique antibodies, dilution-dependent curves in plasma and concentration-dependent curves of full-length proteins for 102 (49%) of the targets are obtained. For 22 protein assays, the longitudinal, interindividual, and technical performance is determined in a set of plasma samples collected from 18 healthy subjects every third month over 1 year. Finally, 14 of these assays are compared with with SIAs composed of other binders, proximity extension assays, and affinity-free targeted mass spectrometry. The workflow provides a multiplexed approach to screen for SIA pairs that suggests using at least three antibodies per target. This design is applicable for a wider range of targets of the plasma proteome, and the assays can be applied for discovery but also to validate emerging candidates derived from other platforms.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- antibodies
- plasma
- sandwich assays
- screening
- secreted proteins
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Häussler, Ragna ...
-
Bendes, Annika
-
Iglesias, Maria ...
-
Sanchez-Rivera, ...
-
Dodig-Crnkovic, ...
-
Byström, Sanna
-
visa fler...
-
Fredolini, Claud ...
-
Birgersson, Elin
-
Dale, Matilda
-
Edfors, Fredrik
-
Fagerberg, Linn
-
Rockberg, Johan
-
Tegel, Hanna
-
Uhlèn, Mathias
-
Qundos, Ulrika
-
Schwenk, Jochen ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
Proteomics
- Av lärosätet
-
Kungliga Tekniska Högskolan