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Cytotoxic effect of...
Cytotoxic effect of crude and purified pectins from Campomanesia xanthocarpa Berg on human glioblastoma cells
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- Amaral, Sarah da Costa (författare)
- Univ Fed Parana, Sect Biol Sci, Postgrad Program Biochem Sci, BR-81531990 Curitiba, PR, Brazil.
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- Barbieri, Shayla Fernanda (författare)
- Univ Fed Parana, Sect Biol Sci, Postgrad Program Biochem Sci, BR-81531990 Curitiba, PR, Brazil.
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- Ruthes, Andrea C. (författare)
- KTH,Glykovetenskap,UF, GCREC, Dept Entomol & Nematol, Wimauma, FL USA.
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- Bark, Juliana Mueller (författare)
- Univ Fed Parana, Sect Biol Sci, Postgrad Program Biochem Sci, BR-81531990 Curitiba, PR, Brazil.
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- Brochado Winnischofer, Sheila Maria (författare)
- Univ Fed Parana, Sect Biol Sci, Postgrad Program Biochem Sci, BR-81531990 Curitiba, PR, Brazil.;Univ Fed Parana, Dept Biochem & Mol Biol, PB 19046, BR-81531980 Curitiba, PR, Brazil.;Univ Fed Parana, Postgrad Program Cellular & Mol Biol, BR-81531980 Curitiba, PR, Brazil.
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- Meira Silveira, Joana Lea (författare)
- Univ Fed Parana, Sect Biol Sci, Postgrad Program Biochem Sci, BR-81531990 Curitiba, PR, Brazil.;Univ Fed Parana, Dept Biochem & Mol Biol, PB 19046, BR-81531980 Curitiba, PR, Brazil.
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Univ Fed Parana, Sect Biol Sci, Postgrad Program Biochem Sci, BR-81531990 Curitiba, PR, Brazil Glykovetenskap (creator_code:org_t)
- ELSEVIER SCI LTD, 2019
- 2019
- Engelska.
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Ingår i: Carbohydrate Polymers. - : ELSEVIER SCI LTD. - 0144-8617 .- 1879-1344. ; 224
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A new source of pectin with a cytotoxic effect on glioblastoma cells is presented. A homogeneous GWP-FP-S fraction (M-w, of 29,170 g mol(-1)) was obtained by fractionating the crude pectin extract (GW) from Campomanesia xanthocarpa pulp. According to the monosaccharide composition, the GWP-FP-S was composed of galacturonic acid (58.8%), arabinose (28.5%), galactose (11.3%) and rhamnose (1.1%), comprising 57.7% of homogalacturonans (HG) and 42.0% of type I rhamnogalacturonans (RG-I). These structures were characterized by chromatographic and spectroscopic methods; GW and GWP-FP-S fractions were evaluated by MIT and crystal violet assays for their cytotoxic effects. Both fractions induced cytotoxicity (15.55-37.65%) with concomitant increase in the cellular ROS levels in human glioblastoma cells at 25-400 mu g mL(-)(1), after 48 h of treatment, whereas no cytotoxicity was observed for normal NIH 3T3 cells. This is the first report of in vitro bioactivity and the first investigation of the antitumor potential of gabiroba pectins.
Ämnesord
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
Nyckelord
- Gabiroba
- Pectin
- NMR analysis
- Glioblastoma cells
- Cytotoxicity
- ROS
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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