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Silkworm Silk Scaff...
Silkworm Silk Scaffolds Functionalized with Recombinant Spider Silk Containing a Fibronectin Motif Promotes Healing of Full-Thickness Burn Wounds
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- Chouhan, Dimple (författare)
- Indian Inst Technol Guwahati, Dept Biosci & Bioengn, Biomat & Tissue Engn Lab, Gauhati 781039, Assam, India.
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- Lohe, Tshewuzo-u (författare)
- Natl Inst Pharmaceut Educ & Res, Dept Pharmacol & Toxicol, Gauhati 781032, Assam, India.
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- Thatikonda, Naresh (författare)
- KTH,Proteinvetenskap
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- Naidu, V. G. M. (författare)
- Natl Inst Pharmaceut Educ & Res, Dept Pharmacol & Toxicol, Gauhati 781032, Assam, India.
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- Hedhammar, My (författare)
- KTH,Proteinteknologi
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- Mandal, Biman B. (författare)
- Indian Inst Technol Guwahati, Dept Biosci & Bioengn, Biomat & Tissue Engn Lab, Gauhati 781039, Assam, India.;Indian Inst Technol Guwahati, Ctr Nanotechnol, Gauhati 781039, Assam, India.
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Indian Inst Technol Guwahati, Dept Biosci & Bioengn, Biomat & Tissue Engn Lab, Gauhati 781039, Assam, India Natl Inst Pharmaceut Educ & Res, Dept Pharmacol & Toxicol, Gauhati 781032, Assam, India. (creator_code:org_t)
- 2019-08-09
- 2019
- Engelska.
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Ingår i: ACS Biomaterials Science & Engineering. - : AMER CHEMICAL SOC. - 2373-9878. ; 5:9, s. 4634-4645
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Full-thickness cutaneous wounds, such as deep burns, are complex wounds that often require surgical interventions. Herein, we show the efficacy of acellular grafts that can be made available off-the-shelf at an affordable cost using silk biomaterials. Silkworm silk fibroin (SF), being a cost-effective and natural biopolymer, provides essential features required for the fabrication of three-dimensional constructs for wound-healing applications. We report the treatment of third-degree burn wounds using a freeze-dried microporous scaffold of Antheraea assama SF (AaSF) functionalized with a recombinant spider silk fusion protein FN-4RepCT (FN-4RC) that holds the fibronectin cell binding motif. In order to examine the healing efficiency of functionalized silk scaffolds, an in vivo burn rat model was used, and the scaffolds were implanted by a one-step grafting procedure. The aim of our work is to investigate the efficacy of the developed acellular silk grafts for treating full-thickness wounds as well as to examine the effect of recombinant spider silk coatings on the healing outcomes. Following 14-day treatment, AaSF scaffolds coated with FN-4RC demonstrated accelerated wound healing when compared to the uncoated counterpart, commercially used DuoDERM dressing patch, and untreated wounds. Histological assessments of wounds over time further confirmed that functionalized silk scaffolds promoted wound healing, showing vascularization and re-epithelialization in the initial phase. In addition, higher extent of tissue remodeling was affirmed by the gene expression study of collagen type I and type III, indicating advanced stage of healing by the silk treatments. Thus, the present study validates the potential of scaffolds of combined silkworm silk and FN-4RC for skin regeneration.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Nyckelord
- silk scaffold
- recombinant spider silk
- burn wounds
- wound healing
- skin regeneration
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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