Sökning: onr:"swepub:oai:DiVA.org:kth-266309" > Effects of the Anti...
Fältnamn | Indikatorer | Metadata |
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000 | 05284naa a2200577 4500 | |
001 | oai:DiVA.org:kth-266309 | |
003 | SwePub | |
008 | 200107s2019 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:142449514 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2663092 URI |
024 | 7 | a https://doi.org/10.3389/fmicb.2019.026322 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1424495142 URI |
040 | a (SwePub)kthd (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Al-Farsi, Hissa M.u Karolinska Inst, Dept Lab Med, Div Clin Microbiol, Stockholm, Sweden.;Minist Hlth, Cent Publ Hlth Labs, Muscat, Oman.4 aut |
245 | 1 0 | a Effects of the Antimicrobial Peptide LL-37 and Innate Effector Mechanisms in Colistin-Resistant Klebsiella pneumoniae With mgrB Insertions |
264 | c 2019-11-14 | |
264 | 1 | b FRONTIERS MEDIA SA,c 2019 |
338 | a print2 rdacarrier | |
500 | a QC 20200107 | |
520 | a Background Colistin is a polypeptide antibiotic drug that targets lipopolysaccharides in the outer membrane of Gram-negative bacteria. Inactivation of the mgrB-gene is a common mechanism behind colistin-resistance in Klebsiella pneumoniae (Kpn). Since colistin is a cyclic polypeptide, it may exhibit cross-resistance with the antimicrobial peptide LL-37, and with other innate effector mechanisms, but previous results are inconclusive. Objective To study potential cross-resistance between colistin and LL-37, as well as with other innate effector mechanisms, and to compare virulence of colistin-resistant and susceptible Kpn strains. Materials/Methods Carbapenemase-producing Kpn from Oman (n = 17) were subjected to antimicrobial susceptibility testing and whole genome sequencing. Susceptibility to colistin and LL-37 was studied. The surface charge was determined by zeta-potential measurements and the morphology of treated bacteria was analyzed with electron microscopy. Bacterial survival was assessed in human whole blood and serum, as well as in a zebrafish infection-model. Results Genome-analysis revealed insertion-sequences in the mgrB gene, as a cause of colistin resistance in 8/17 isolates. Colistin-resistant (Col-R) isolates were found to be more resistant to LL-37 compared to colistin-susceptible (Col-S) isolates, but only at concentrations >= 50 mu g/ml. There was no significant difference in surface charge between the isolates. The morphological changes were similar in both Col-R and Col-S isolates after exposure to LL-37. Finally, no survival difference between the Col-R and Col-S isolates was observed in whole blood or serum, or in zebrafish embryos. Conclusion Cross-resistance between colistin and LL-37 was observed at elevated concentrations of LL-37. However, Col-R and Col-S isolates exhibited similar survival in serum and whole blood, and in a zebrafish infection-model, suggesting that cross-resistance most likely play a limited role during physiological conditions. However, it cannot be ruled out that the observed cross-resistance could be relevant in conditions where LL-37 levels reach high concentrations, such as during infection or inflammation. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicin0 (SwePub)3022 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicine0 (SwePub)3022 hsv//eng |
653 | a cross-resistance | |
653 | a colistin | |
653 | a LL-37 | |
653 | a innate immunity | |
653 | a zeta potential | |
653 | a whole blood killing assay | |
653 | a serum killing assay | |
653 | a zebrafish | |
700 | 1 | a Al-Adwani, Salmau Karolinska Institutet4 aut |
700 | 1 | a Ahmed, Sultanu Karolinska Inst, Dept Lab Med, Div Clin Microbiol, Stockholm, Sweden.4 aut |
700 | 1 | a Vogt, Carmenu KTH,Biomedicinsk fysik och röntgenfysik,Albanova VinnExcellence Center for Protein Technology, ProNova4 aut0 (Swepub:kth)u1iann4b |
700 | 1 | a Ambikan, Anoop T.u Karolinska Institutet4 aut |
700 | 1 | a Leber, Annau Karolinska Inst, Dept Lab Med, Div Clin Microbiol, Stockholm, Sweden.4 aut |
700 | 1 | a Al-Jardani, Aminau Minist Hlth, Cent Publ Hlth Labs, Muscat, Oman.4 aut |
700 | 1 | a Al-Azri, Salehu Minist Hlth, Cent Publ Hlth Labs, Muscat, Oman.4 aut |
700 | 1 | a Al-Muharmi, Zakariyau Sultan Qaboos Univ, Coll Med & Hlth Sci, Dept Microbiol & Immunol, Muscat, Oman.4 aut |
700 | 1 | a Toprak, Muhammet,d 1973-u KTH,Biomedicinsk fysik och röntgenfysik,Albanova VinnExcellence Center for Protein Technology, ProNova4 aut0 (Swepub:kth)u1u3m5a2 |
700 | 1 | a Giske, Christian G.u Karolinska Institutet4 aut |
700 | 1 | a Bergman, Peteru Karolinska Institutet4 aut |
710 | 2 | a Karolinska Institutetb Karolinska Inst, Dept Lab Med, Div Clin Microbiol, Stockholm, Sweden.;Minist Hlth, Cent Publ Hlth Labs, Muscat, Oman.4 org |
773 | 0 | t Frontiers in Microbiologyd : FRONTIERS MEDIA SAg 10q 10x 1664-302X |
856 | 4 | u https://www.frontiersin.org/articles/10.3389/fmicb.2019.02632/pdf |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-266309 |
856 | 4 8 | u https://doi.org/10.3389/fmicb.2019.02632 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:142449514 |
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