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Layer by layer coat...
Layer by layer coating for bio-functionalization of additively manufactured meta-biomaterials
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Amin Yavari, S. (författare)
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Croes, M. (författare)
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Akhavan, B. (författare)
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Jahanmard, F. (författare)
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Eigenhuis, C. C. (författare)
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- Dadbakhsh, Sasan (författare)
- KTH,Skolan för industriell teknik och management (ITM)
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Vogely, H. C. (författare)
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Bilek, M. M. (författare)
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Fluit, A. C. (författare)
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Boel, C. H. E. (författare)
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van der Wal, B. C. H. (författare)
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Vermonden, T. (författare)
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Weinans, H. (författare)
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Zadpoor, A. A. (författare)
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(creator_code:org_t)
- Elsevier BV, 2020
- 2020
- Engelska.
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Ingår i: Additive Manufacturing. - : Elsevier BV. - 2214-8604 .- 2214-7810. ; 32
- Relaterad länk:
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https://doi.org/10.1...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Additive manufacturing has facilitated fabrication of complex and patient-specific metallic meta-biomaterials that offer an unprecedented collection of mechanical, mass transport, and biological properties as well as a fully interconnected porous structure. However, applying meta-biomaterials for addressing unmet clinical needs in orthopedic surgery requires additional surface functionalities that should be induced through tailor-made coatings. Here, we developed multi-functional layer-by-layer coatings to simultaneously prevent implant-associated infections and stimulate bone tissue regeneration. We applied multiple layers of gelatin- and chitosan-based coatings containing either bone morphogenetic protein (BMP)-2 or vancomycin on the surface of selective laser melted porous structures made from commercial pure Titanium (CP Ti) and designed using a triply periodic minimal surface (i.e., sheet gyroid). The additive manufacturing process resulted in a porous structure and met the the design values comparatively. X-ray photoelectron spectroscopy spectra confirmed the presence and composition of the coating layers. The release profiles showed a continued release of both vancomycin and BMP-2 for 2–3 weeks. Furthermore, the developed meta-biomaterials exhibited a very strong antibacterial behavior with up to 8 orders of magnitude reduction in both planktonic and implant-adherent bacteria and no signs of biofilm formation. The osteogenic differentiation of mesenchymal stem cells was enhanced, as shown by two-fold increase in the alkaline phosphatase activity and up to four-fold increase in the mineralization of all experimental groups containing BMP-2. Eight-week subcutaneous implantation in vivo showed no signs of a foreign body response, while connective tissue ingrowth was promoted by the layer-by-layer coating. These results unequivocally confirm the superior multi-functional performance of the developed biomaterials.
Nyckelord
- Meta-biomaterials
- Metal 3D printing
- Layer-by-layer surface bio-functionalization
- Multi-functional coatings
Publikations- och innehållstyp
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- art (ämneskategori)
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Till lärosätets databas
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Amin Yavari, S.
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Croes, M.
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Akhavan, B.
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Jahanmard, F.
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Eigenhuis, C. C.
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Dadbakhsh, Sasan
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visa fler...
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Vogely, H. C.
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Bilek, M. M.
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Fluit, A. C.
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Boel, C. H. E.
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van der Wal, B. ...
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Vermonden, T.
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Weinans, H.
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Zadpoor, A. A.
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visa färre...
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Additive Manufac ...
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Kungliga Tekniska Högskolan