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Sökning: onr:"swepub:oai:DiVA.org:kth-27079" > Response of Bone an...

  • Schander, Kerstin (författare)

Response of Bone and Periodontal Ligament Cells to Biodegradable Polymer Scaffolds In Vitro

  • Artikel/kapitelEngelska2010

Förlag, utgivningsår, omfång ...

  • 2010-10-18
  • SAGE Publications,2010
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:kth-27079
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-27079URI
  • https://doi.org/10.1177/0883911510383684DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • QC 20101210
  • In this in vitro study, the initial response of human periodontal ligament (PDL) cells and alveolar osteoblast-like cells (HOB) to three biodegradable polymers with varying pore size and different mechanical properties were evaluated. Scaffolds were synthesized from poly(L-lactide), [poly(LLA)], poly(L-lactide-co-1,5-dioxepan-2-one), [poly(LLA-co-DXO)], poly(L-lactide-co-epsilon-caprolactone), and [poly(LLA-co-CL)] with pore sizes greater or less than 90 mm by salt leaching. Cells were obtained from patients undergoing routine oral surgery. After 2-4 passages, the cells were grown on scaffolds and in culture plates (control) for 3 h (PDL cells), 3 days (PDL cells and HOB), 10 and 14 days (HOB), respectively. The cellular morphology and spreading were determined by scanning electron microscopy (SEM) and the attachment and proliferation were evaluated by MTT assays. The SEM images revealed heterogeneous cellular morphology and good spreading. Cellular attachment and proliferation were significantly higher on poly(LLA-co-DXO) and poly(LLA-co-CL) than on poly(LLA) scaffolds (p = 0.003) and highest for poly(LLA-co-DXO). Expression of bone formation markers, collagen-I (COL-I), transforming growth factor-beta(1) (TGF-beta(1)), and osteocalcin (OCN), was determined by ELISA. The expression of COL-1 was similar for HOB and PDL cells, but significantly higher for pore size >90 mm while the HOB expression of TGF-beta(1) and OCN was greater on poly(LLA-co-CL) and poly(LLA-co-DXO) than on poly(LLA) scaffolds.

Ämnesord och genrebeteckningar

  • alveolar osteoblasts
  • aliphatic polyesters
  • porous
  • cell attachment
  • PDL-cells
  • bone growth
  • collagen expression
  • poly(LLA-co-DXO)
  • poly(LLA-co-CL)
  • poly(LLA) scaffolds
  • periodontal ligament cells
  • TECHNOLOGY
  • TEKNIKVETENSKAP

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Arvidson, Kristina (författare)
  • Mustafa, Kamal (författare)
  • Hellem, Endre (författare)
  • Bolstad, Anne Isine (författare)
  • Finne Wistrand, AnnaKTH,Polymerteknologi(Swepub:kth)u17gwfv7 (författare)
  • Albertsson, Ann-ChristineKTH,Polymerteknologi(Swepub:kth)u1mpsp3u (författare)
  • KTHPolymerteknologi (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Journal of bioactive and compatible polymers (Print): SAGE Publications25:6, s. 584-6020883-91151530-8030

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