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Click chemical asse...
Click chemical assembly and validation of bio-functionalized superparamagnetic hybrid microspheres
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- Saladino, Giovanni (författare)
- KTH,Biomedicinsk fysik och röntgenfysik
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- Hamawandi, Bejan, PhD (författare)
- KTH,Biomedicinsk fysik och röntgenfysik
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- Vogt, Carmen (författare)
- KTH,Biomedicinsk fysik och röntgenfysik,Albanova VinnExcellence Center for Protein Technology, ProNova
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- Rajarao, Gunaratna Kuttuva (författare)
- KTH,Industriell bioteknologi
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- Toprak, Muhammet, 1973- (författare)
- KTH,Biomedicinsk fysik och röntgenfysik
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(creator_code:org_t)
- 2020-02-14
- 2020
- Engelska.
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Ingår i: Applied Nanoscience. - : Springer Nature. - 2190-5509 .- 2190-5517.
- Relaterad länk:
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https://doi.org/10.1...
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https://link.springe...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Surface derivatized magnetic nanoparticles have been commonly used for magnetic separation. Facile mechanisms are needed to be developed for the design of bio-functionalized magnetic hybrid materials, where the surfaces can be re-generated for the re-use of the developed platforms. Superparamagnetic iron oxide nanoparticles with a diameter below 10 nm were synthesized via a novel microwave-assisted hydrothermal method in the presence of citrate ions, which allowed to obtain uniform and negatively charged nanoparticles. These were then coupled with Poly-l-lysine (PLL), forming micrometer-sized self-assembled spherical entities. Cross-linking the PLL within these microspheres with glutaraldehyde stabilized them chemically and mechanically. The active bio-functionality was introduced by a protein grafting methodology, using m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester (SMBS). The Moringa oleifera Coagulant Protein (MOCP) from a seed extract was employed for its characteristic coagulation activity. The performance of the MOCP functionalized microspheres was evaluated as a function of turbidity removal of problematic colloidal clay from water via magnetic separation, resulting in over 80% of activity within 15 min. Surface of these hybrid materials can be re-generated by treatment with alcohol, allowing their easy magnetic separation and re-use. The rapid and strong response with tunable magnetic property makes these hybrid microspheres a powerful tool for many potential applications, due to the general applicability of the developed methodology.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
Nyckelord
- Bio-functionalization
- Hybrid platform
- Protein grafting
- SPIONs
- Superparamagnetic microspheres
- Turbidity removal
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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