Nanoassembled Peptide Biosensors for Rapid Detection of Matrilysin Cancer Biomarker

• Early detection of cancer is likely to be one of the most effective means of reducing the cancer mortality rate. Hence, simple and ultra-quick methods for noninvasive detection of early-stage tumors are highly sought-after. In this study, a nanobiosensing platform with a rapid response time of nearly 30 s is introduced for the detection of matrilysin—the salivary gland cancer biomarker—with a limit of detection as low as 30 nm. This sensing platform is based on matrilysin-digestible peptides that bridge gold nanoparticle (AuNPs) cores (≈30–50 nm) and carbon quantum dot (CDs) satellites (≈9 nm). A stepwise synthesis procedure is used for self-assembly of AuNP-peptide-CDs, ensuring their long-term stability. The AuNP-peptide-CDs produce ideal optical signals, with noticeable fluorescence quenching effects. Upon peptide cleavage by matrilysin, CDs leave the surface of AuNPs, resulting in ultra-fast detectable violet and visible fluorescent signals.

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Kemiteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Chemical Engineering (hsv//eng)

Nyckelord

hybrid architectures

matrilysin

nanobiosensors

optical sensing

sensing platforms

tumor detection

Biomarkers

Carbon Quantum Dots

Diseases

Fluorescence

Gold nanoparticles

Quenching

Self assembly

Semiconductor quantum dots

Tumors

Nano-biosensors

Peptides

Publikations- och innehållstyp

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