Bioaugmented mixed culture fermentation by Clostridium aceticum to enhance acetic acid dominant volatile fatty acids production from dairy industry wastewater

•  By increasing volatile fatty acids (VFA) production efficiency via fermentation can lead to shifting from industrial wastewater treatment plants to biorefineries. In the current paper, acetic acid dominant VFA mixture production by bioaugmentation of mixed culture with Clostridium aceticum was aimed. The maximum acetic acid concentration was increased 10 times in the bioaugmented reactor (1170±18 mgCOD/L) than the control reactor (122±9 mgCOD/L). Additionally, the highest total VFA production was 5 times higher in bioaugmented reactor than the control reactor. Nevertheless, the bioaugmentation did not affect the propionic acid dominancy in the VFA mixture. The correlation analysis stated that the copy gene number of C. aceticum was highly correlated with acetic acid (p<0.05) and iso-valeric acid (p<0.01). The cycle analysis stated that 6 hours was adequate retention time to have the same acetic acid and total VFA production efficiency instead of 24 hours. 

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Miljöbioteknik -- Annan miljöbioteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Environmental Biotechnology -- Other Environmental Biotechnology (hsv//eng)

Nyckelord

Bioaugmentation

mixed culture fermentation

acetic acid

Clostridium aceticum

anaerobic digestion

dairy industry wastewater

Q-PCR

Bioteknologi

Biotechnology

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