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Enrichment and Iden...
Enrichment and Identification of Lignin-Carbohydrate Complexes in Softwood Extract
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- de Carvalho, Danila Morais (författare)
- Univ Helsinki, Dept Food & Nutr, Fac Agr & Forestry, FI-00014 Helsinki, Finland.
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- Lahtinen, Maarit H. (författare)
- Univ Helsinki, Dept Food & Nutr, Fac Agr & Forestry, FI-00014 Helsinki, Finland.
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- Lawoko, Martin (författare)
- KTH,Fiber- och polymerteknologi
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- Mikkonen, Kirsi S. (författare)
- Univ Helsinki, Dept Food & Nutr, Fac Agr & Forestry, FI-00014 Helsinki, Finland.;Univ Helsinki, Helsinki Inst Sustainabil Sci HELSUS, FI-00014 Helsinki, Finland.
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Univ Helsinki, Dept Food & Nutr, Fac Agr & Forestry, FI-00014 Helsinki, Finland Fiber- och polymerteknologi (creator_code:org_t)
- 2020-07-28
- 2020
- Engelska.
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Ingår i: ACS Sustainable Chemistry and Engineering. - : American Chemical Society (ACS). - 2168-0485. ; 8:31, s. 11795-11804
- Relaterad länk:
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https://doi.org/10.1...
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https://pubs.acs.org...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Lignin-carbohydrate complexes (LCCs) are hybrid structures containing covalently linked moieties of lignin and carbohydrates. The structure and behavior of LCCs affect both industrial processes and practical applications of lignocellulosic biomass. However, the identification of phenylglycoside, benzylether, and gamma (gamma)-ester LCC bonds in lignocellulosic biomass is limited due to their relatively low abundance compared to plain carbohydrate and lignin structures. Herein, we enriched the LCC bonds in softwood galactoglucomannan (GGM)-rich extract fractionated by (1) a solvent (ethanol), (2) enzymes, and (3) physical techniques. Two-dimensional nuclear magnetic resonance (NMR) spectroscopy analysis was used to identify the LCC bonds. Phenylglycoside and benzylether bonds were concentrated in the ethanol-soluble GGM fractions. A benzylether bond was concentrated into GGM fractions containing larger molecules (>500 Da) through physical techniques. The gamma-ester bond was identified in all studied GGM fractions, which is explained by its stability and possible presence in residual xylan. In summary, we demonstrated the potential of the suggested techniques to enrich LCC bonds in softwood extract and improve LCC identification. Such techniques may also enable further studies on the structure and functionality of LCC bonds and open new prospects in the engineering of biomolecules.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Pappers-, massa- och fiberteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Paper, Pulp and Fiber Technology (hsv//eng)
Nyckelord
- Antisolvent separation
- Galactoglucomannan (GGM)
- Nanofiltration
- Nuclear magnetic resonance (NMR) spectroscopy
- Semi-simultaneous enzymatic hydrolysis (SSEH)
- Spruce wood
- Ultracentrifugation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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