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Mapping the nucleol...
Mapping the nucleolar proteome reveals a spatiotemporal organization related to intrinsic protein disorder
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- Stenström, Lovisa (författare)
- KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab
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- Mahdessian, Diana (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Cellulär och klinisk proteomik,Sch Engn Sci Chem Biotechnol & Hlth, Sci Life Lab, Stockholm, Sweden.
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- Gnann, Christian (författare)
- KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Chan Zuckerberg Biohub, San Francisco, CA 94158 USA.
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- Cesnik, Anthony J. (författare)
- Chan Zuckerberg Biohub, San Francisco, CA 94158 USA.;Stanford Univ, Dept Genet, Stanford, CA 94305 USA.
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- Ouyang, Wei (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Cellulär och klinisk proteomik
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- Leonetti, Manuel D. (författare)
- Chan Zuckerberg Biohub, San Francisco, CA 94158 USA.
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- Uhlén, Mathias (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Systembiologi
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- Cuylen-Haering, Sara (författare)
- European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, Germany.
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- Thul, Peter (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Cellulär och klinisk proteomik
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- Lundberg, Emma (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Cellulär och klinisk proteomik,Chan Zuckerberg Biohub, San Francisco, CA 94158 USA.;Stanford Univ, Dept Genet, Stanford, CA 94305 USA.
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(creator_code:org_t)
- 2020-08-03
- 2020
- Engelska.
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Ingår i: Molecular Systems Biology. - : Wiley. - 1744-4292 .- 1744-4292. ; 16:8
- Relaterad länk:
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https://doi.org/10.1...
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https://onlinelibrar...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The nucleolus is essential for ribosome biogenesis and is involved in many other cellular functions. We performed a systematic spatiotemporal dissection of the human nucleolar proteome using confocal microscopy. In total, 1,318 nucleolar proteins were identified; 287 were localized to fibrillar components, and 157 were enriched along the nucleoplasmic border, indicating a potential fourth nucleolar subcompartment: the nucleoli rim. We found 65 nucleolar proteins (36 uncharacterized) to relocate to the chromosomal periphery during mitosis. Interestingly, we observed temporal partitioning into two recruitment phenotypes: early (prometaphase) and late (after metaphase), suggesting phase-specific functions. We further show that the expression ofMKI67 is critical for this temporal partitioning. We provide the first proteome-wide analysis of intrinsic protein disorder for the human nucleolus and show that nucleolar proteins in general, and mitotic chromosome proteins in particular, have significantly higher intrinsic disorder level compared to cytosolic proteins. In summary, this study provides a comprehensive and essential resource of spatiotemporal expression data for the nucleolar proteome as part of the Human Protein Atlas.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- human protein atlas
- intrinsic protein disorder
- nucleolus
- perichromosomal layer
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Stenström, Lovis ...
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Mahdessian, Dian ...
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Gnann, Christian
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Cesnik, Anthony ...
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Ouyang, Wei
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Leonetti, Manuel ...
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visa fler...
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Uhlén, Mathias
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Cuylen-Haering, ...
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Thul, Peter
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Lundberg, Emma
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visa färre...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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Molecular System ...
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Kungliga Tekniska Högskolan