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Sökning: onr:"swepub:oai:DiVA.org:kth-285728" > Assessment of antid...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00006124naa a2200625 4500
001oai:DiVA.org:kth-285728
003SwePub
008201123s2020 | |||||||||||000 ||eng|
009oai:slubar.slu.se:108433
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2857282 URI
0247 a https://doi.org/10.1186/s12906-020-03035-x2 DOI
0247 a https://res.slu.se/id/publ/1084332 URI
040 a (SwePub)kthd (SwePub)slu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Mahmood, Rashidu Quaid I Azam Univ, Fac Biol Sci, Dept Biochem, Islamabad, Pakistan.;Natl Inst Hlth, Drugs Control & Tradit Medicines Div, Islamabad, Pakistan.4 aut
2451 0a Assessment of antidiabetic potential and phytochemical profiling of Rhazya stricta root extracts
264 c 2020-09-29
264 1b Springer Nature,c 2020
338 a print2 rdacarrier
500 a QC 20201123
520 a Background: Diabetes mellitus is a chronic disease characterized by hyperglycemia that may occur due to genetic, environmental or lifestyle factors. Natural remedies have been used to treat diabetes since long and many antidiabetic compounds of varied efficacies have been isolated from medicinal plants. Rhazya stricta has been used for decades for the treatment of diabetes mellitus and associated ailments. Considering the folkloric use of R. stricta against diabetes, it was aimed to investigate the effectiveness of its root extracts against diabetes through in vitro assays and in vivo studies using animal model along with phytochemical profiling through GCMS. Methods: Various fractions of Rhazya stricta obtained through column chromatography were evaluated for a variety of assays including a-glucosidase, Dipeptidyl peptidase-IV (DPP-IV), beta-secretase and Glucagon-like peptide-1 (GLP-1) secretion studies. For the in vivo studies the alloxan-induced diabetic mice were treated with root extracts and blood glucose levels, HbA1C, and other biochemical markers along with the histological study of the liver were done. The phytochemical identification was performed using an Agilent 7890B GC coupled to a 7010 Triple Quadrupole (MS/MS) system. GraphPad Prism software version 5.01 was used for statistical analysis. Results: Majority of the extract fractions showed excellent results against diabetes by inhibiting enzymes DPP-IV (Up to 61%) and beta-secretase (Up to 83%) with IC50s 979 pg/ml and 169 mu g/ml respectively with increase in the GLP1 secretion. The results of in vivo studies indicated a marked reduction in blood glucose and HbA1c levels along with positive effects on other parameters like lipid profile, liver functions and renal functions of extract-treated mice as compared to control. The histological examination of the liver demonstrated hepatoprotective effects against diabetes led changes and various classes of phytochemicals were also identified through GCMS in different fractions. Conclusion: The results revealed strong antidiabetic activity of R. stricta root with the potential to protect body organs against diabetic changes. Moreover, a variety of phytochemicals has also been identified through GCMS that might be responsible for the antidiabetic potential of Rhazya stricta root.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Farmakologi och toxikologi0 (SwePub)301022 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Pharmacology and Toxicology0 (SwePub)301022 hsv//eng
653 a Diabetes mellitus
653 a beta-Secretase
653 a DPP-IV
653 a GLP-1 secretion
653 a In vivo studies
653 a Alloxan induced mice
653 a GC-MS
7001 a Kayani, Waqas Khanu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtförädling,Department of Plant Breeding4 aut0 (Swepub:slu)78984
7001 a Ahmed, Tanveeru Quaid I Azam Univ, Fac Biol Sci, Dept Biochem, Islamabad, Pakistan.4 aut
7001 a Malik, Farnazu Natl Inst Hlth, Drugs Control & Tradit Medicines Div, Islamabad, Pakistan.4 aut
7001 a Hussain, Shahzadu Natl Inst Hlth, Drugs Control & Tradit Medicines Div, Islamabad, Pakistan.4 aut
7001 a Ashfaq, Muhammadu Natl Inst Hlth, Drugs Control & Tradit Medicines Div, Islamabad, Pakistan.4 aut
7001 a Ali, Hussainu Natl Inst Hlth, Anim House, Islamabad, Pakistan.4 aut
7001 a Rubnawaz, Saminau Quaid I Azam Univ, Fac Biol Sci, Dept Biochem, Islamabad, Pakistan.4 aut
7001 a Green, Brian D.u Queens Univ Belfast, Inst Global Food Secur, Sch Biol Sci, Belfast, Antrim, North Ireland.4 aut
7001 a Calderwood, Danielleu Queens Univ Belfast, Inst Global Food Secur, Sch Biol Sci, Belfast, Antrim, North Ireland.4 aut
7001 a Kenny, Owenu Queens Univ Belfast, Inst Global Food Secur, Sch Biol Sci, Belfast, Antrim, North Ireland.4 aut
7001 a Rivera, Gerardo A.u Queens Univ Belfast, Inst Global Food Secur, Sch Biol Sci, Belfast, Antrim, North Ireland.4 aut
7001 a Mirza, Bushrau Quaid I Azam Univ, Fac Biol Sci, Dept Biochem, Islamabad, Pakistan.4 aut
7001 a Rasheed, Faizau KTH,Polymera material4 aut0 (Swepub:kth)u19xk6vx
7102 a Quaid I Azam Univ, Fac Biol Sci, Dept Biochem, Islamabad, Pakistan.;Natl Inst Hlth, Drugs Control & Tradit Medicines Div, Islamabad, Pakistan.b Institutionen för växtförädling4 org
7102 a Sveriges lantbruksuniversitet
7730 t BMC Complementary Medicine and Therapiesd : Springer Natureg 20:1q 20:1x 2662-7671
8564 u https://doi.org/10.1186/s12906-020-03035-xy Fulltext
8564 u https://bmccomplementmedtherapies.biomedcentral.com/track/pdf/10.1186/s12906-020-03035-x
8564 u https://pub.epsilon.slu.se/id/eprint/21959/contentsx primaryx Raw objectx freey FULLTEXT
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-285728
8564 8u https://doi.org/10.1186/s12906-020-03035-x
8564 8u https://res.slu.se/id/publ/108433

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