Sökning: onr:"swepub:oai:DiVA.org:kth-291752" > Integrated Single C...
Fältnamn | Indikatorer | Metadata |
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000 | 03393nam a2200445 4500 | |
001 | oai:DiVA.org:kth-291752 | |
003 | SwePub | |
008 | 210318| | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2917522 URI |
040 | a (SwePub)kth | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a vet2 swepub-contenttype |
072 | 7 | a ovr2 swepub-publicationtype |
100 | 1 | a Carlberg, Konstantinu KTH,Genteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u15np845 |
245 | 1 0 | a Integrated Single Cell and Spatial Transcriptomics Reveal Autoreactive Differentiated B Cells in Joints of Early Rheumatoid Arthritis |
338 | a print2 rdacarrier | |
500 | a QC 20210322 | |
520 | a Rheumatoid Arthritis (RA) is a prevalent autoimmune disease characterized by inflammation of peripheral joints. Patients can be subdivided by the presence or absence of Rheumatoid Factor and anti-citrullinated protein antibodies (ACPA) in their circulation. Inflammation of the joint tissue is associated with infiltration of leukocytes from the blood, which can result in generation of lymphoid structures composed of B and T cells. Previous studies have shown that both memory B cells and antibody-secreting plasma cells populate the rheumatic joint tissue when captured from established and often long-standing disease. However, it has remained unclear, whether these cells are autoreactive and whether the associated lymphoid structures are present at the site of inflammation already at the time of diagnosis. Here, we used an integrated single cell and spatial transcriptomic approach to study B and plasma cells in synovial tissue of ACPA- and ACPA+ RA patients at this early time point. We found evidence for T cell help to B cells and presence of memory B and plasma cell pools in ACPA- as well as in ACPA+ RA. Our results demonstrated common supportive microenvironments in both patient subgroups, clonal relationships between the memory B and plasma cell pools and autoreactivity within the plasma cell compartment. These findings challenge our understanding of the dynamics of local adaptive immune responses in the RA joint of ACPA- and ACPA+ patients at the time of diagnosis, with direct implications for B and T cell targeting therapies for both patient subgroups. | |
650 | 7 | a NATURVETENSKAPx Biologix Immunologi0 (SwePub)106052 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Immunology0 (SwePub)106052 hsv//eng |
653 | a Bioteknologi | |
653 | a Biotechnology | |
700 | 1 | a Hardt, Uta4 aut |
700 | 1 | a af Klint, Erik4 aut |
700 | 1 | a Sahlström, Peter4 aut |
700 | 1 | a Larsson, Ludvigu KTH,Genteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u13lw4hm |
700 | 1 | a van Vollenhoven, Annika4 aut |
700 | 1 | a Hernandez Machado, Susana4 aut |
700 | 1 | a Israelsson, Lena4 aut |
700 | 1 | a Amara, Khaled4 aut |
700 | 1 | a Chemin, Karine4 aut |
700 | 1 | a Korotkova, Marina4 aut |
700 | 1 | a Karlsson Hedestam, Gunilla4 aut |
700 | 1 | a Catrina, Anca4 aut |
700 | 1 | a Teichmann, Sarah4 aut |
700 | 1 | a Ståhl, Patrik,c Dr.u KTH,Science for Life Laboratory, SciLifeLab,Genteknologi4 aut0 (Swepub:kth)u1rstx38 |
700 | 1 | a Malmström, Vivianne4 aut |
710 | 2 | a KTHb Genteknologi4 org |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-291752 |
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