Sökning: onr:"swepub:oai:DiVA.org:kth-291802" >
Production of Struc...
Production of Structurally Defined Chito-Oligosaccharides with a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli
-
- Li, Jing (författare)
- KTH,Glykovetenskap,Yt- och korrosionsvetenskap
-
- Wang, Damao (författare)
- KTH,Glykovetenskap
-
- Chang, Shu-Chieh (författare)
- KTH,Glykovetenskap
-
visa fler...
-
- Liang, Pi-Hui (författare)
- Institute of Chemistry, Academia Sinica, 128 Academia Road Sec. 2, Nankang, Taipei 115, Taiwan
-
- Srivastava, Vaibhav (författare)
- KTH,Glykovetenskap
-
- Guu, Shih-Yun (författare)
- Institute of Chemistry, Academia Sinica, 128 Academia Road Sec. 2, Nankang, Taipei 115, Taiwan
-
- Shie, Jiun-Jie (författare)
- Institute of Chemistry, Academia Sinica, 128 Academia Road Sec. 2, Nankang, Taipei 115, Taiwan
-
- Khoo, Kay-Hooi (författare)
- Institute of Chemistry, Academia Sinica, 128 Academia Road Sec. 2, Nankang, Taipei 115, Taiwan
-
- Bulone, Vincent (författare)
- KTH,Glykovetenskap
-
- Hsieh, Yves S. Y. (författare)
- KTH,Glykovetenskap,Wallenberg Wood Science Center,Institute of Chemistry, Academia Sinica, 128 Academia Road Sec. 2, Nankang, Taipei 115, Taiwan
-
visa färre...
-
(creator_code:org_t)
- 2021-03-10
- 2021
- Engelska.
-
Ingår i: Journal of Agricultural and Food Chemistry. - : American Chemical Society. - 0021-8561 .- 1520-5118. ; 69:11, s. 3371-3379
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://doi.org/10.1...
-
https://kth.diva-por... (primary) (Raw object)
-
https://pubs.acs.org...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Partially acetylated chito-oligosaccharides (paCOSs) are bioactive compounds with potential medical applications. Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain. The production of structurally defined paCOSs in a purified form is highly desirable to better understand the structure/bioactivity relationship of these oligosaccharides. Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end. We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(n)GlcNAc1, is due to a subsite specificity toward GlcN residues at the −2, −3, and −4 positions of the partially acetylated chitosan substrates. In addition, the enzyme is stable, as evidenced by its long shelf life, and active over a large temperature range, which is of high interest for potential use in industrial processes. It exhibits a kcatof 67.2 s–1 on partially acetylated chitosan substrates. When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured. PpChi represents an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure and the expansion of the relatively small library of chito-oligosaccharides currently available.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Li, Jing
-
Wang, Damao
-
Chang, Shu-Chieh
-
Liang, Pi-Hui
-
Srivastava, Vaib ...
-
Guu, Shih-Yun
-
visa fler...
-
Shie, Jiun-Jie
-
Khoo, Kay-Hooi
-
Bulone, Vincent
-
Hsieh, Yves S. Y ...
-
visa färre...
- Om ämnet
-
- TEKNIK OCH TEKNOLOGIER
-
TEKNIK OCH TEKNO ...
-
och Industriell biot ...
-
och Biokatalys och e ...
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
Journal of Agric ...
- Av lärosätet
-
Kungliga Tekniska Högskolan