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Quantitative conver...
Quantitative conversion of biomass in giant DNA virus infection
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- Kördel, Mikael (författare)
- KTH,Biomedicinsk fysik och röntgenfysik,Albanova VinnExcellence Center for Protein Technology, ProNova,Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, Husargatan 3,Box 596, S-75124 Uppsala, Sweden.,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & XRay Phys, S-10691 Stockholm, Sweden
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- Svenda, Martin (författare)
- Uppsala universitet,KTH,Biomedicinsk fysik och röntgenfysik,Albanova VinnExcellence Center for Protein Technology, ProNova,Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, Husargatan 3,Box 596, S-75124 Uppsala, Sweden.,Molekylär biofysik,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & XRay Phys, S-10691 Stockholm, Sweden
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- Reddy, Hemanth K. N. (författare)
- Uppsala universitet,Molekylär biofysik
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- Fogelqvist, Emelie (författare)
- KTH,Biomedicinsk fysik och röntgenfysik,Albanova VinnExcellence Center for Protein Technology, ProNova,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & XRay Phys, S-10691 Stockholm, Sweden
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- Arsana, Komang G.Y. (författare)
- KTH,Biomedicinsk fysik och röntgenfysik,Albanova VinnExcellence Center for Protein Technology, ProNova,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & XRay Phys, S-10691 Stockholm, Sweden
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- Hamawandi, Bejan, PhD (författare)
- KTH,Biomedicinsk fysik och röntgenfysik,Albanova VinnExcellence Center for Protein Technology, ProNova,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & XRay Phys, S-10691 Stockholm, Sweden
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- Toprak, Muhammet, 1973- (författare)
- KTH,Tillämpad fysik,Albanova VinnExcellence Center for Protein Technology, ProNova,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & XRay Phys, S-10691 Stockholm, Sweden
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- Hertz, Hans (författare)
- KTH,Biomedicinsk fysik och röntgenfysik,Albanova VinnExcellence Center for Protein Technology, ProNova,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & XRay Phys, S-10691 Stockholm, Sweden
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- Sellberg, Jonas A. (författare)
- KTH,Biomedicinsk fysik och röntgenfysik,Albanova VinnExcellence Center for Protein Technology, ProNova,Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, Husargatan 3,Box 596, S-75124 Uppsala, Sweden.,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & XRay Phys, S-10691 Stockholm, Sweden
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(creator_code:org_t)
- 2021-03-03
- 2021
- Engelska.
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Ingår i: Scientific Reports. - : Springer Nature. - 2045-2322. ; 11:1
- Relaterad länk:
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https://doi.org/10.1...
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https://www.nature.c...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Bioconversion of organic materials is the foundation of many applications in chemical engineering, microbiology and biochemistry. Herein, we introduce a new methodology to quantitatively determine conversion of biomass in viral infections while simultaneously imaging morphological changes of the host cell. As proof of concept, the viral replication of an unidentified giant DNA virus and the cellular response of an amoebal host are studied using soft X-ray microscopy, titration dilution measurements and thermal gravimetric analysis. We find that virions produced inside the cell are visible from 18 h post infection and their numbers increase gradually to a burst size of 280-660 virions. Due to the large size of the virion and its strong X-ray absorption contrast, we estimate that the burst size corresponds to a conversion of 6-12% of carbonaceous biomass from amoebal host to virus. The occurrence of virion production correlates with the appearance of a possible viral factory and morphological changes in the phagosomes and contractile vacuole complex of the amoeba, whereas the nucleus and nucleolus appear unaffected throughout most of the replication cycle.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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