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  • Betts, Natalie S.Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia. (författare)

Identification and spatio-temporal expression analysis of barley genes that encode putative modular xylanolytic enzymes

  • Artikel/kapitelEngelska2021

Förlag, utgivningsår, omfång ...

  • Elsevier BV,2021
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:kth-297735
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-297735URI
  • https://doi.org/10.1016/j.plantsci.2020.110792DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • QC 20210624
  • Arabinoxylans are cell wall polysaccharides whose re-modelling and degradation during plant development are mediated by several classes of xylanolytic enzymes. Here, we present the identification and new annotation of twelve putative (1,4)-13-xylanase and six 13-xylosidase genes, and their spatio-temporal expression patterns during vegetative and reproductive growth of barley (Hordeum vulgare cv. Navigator). The encoded xylanase proteins are all predicted to contain a conserved carbohydrate-binding module (CBM) and a catalytic glycoside hydrolase (GH) 10 domain. Additional domains in some xylanases define three discrete phylogenetic clades: one clade contains proteins with an additional N-terminal signal sequence, while another clade contains proteins with multiple CBMs. Homology modelling revealed that all fifteen xylanases likely contain a third domain, a 13-sandwich folded from two non-contiguous sequence segments that bracket the catalytic GH domain, which may explain why the full length protein is required for correct folding of the active enzyme. Similarly, predicted xylosidase proteins share a highly conserved domain structure, each with an N-terminal signal peptide, a split GH 3 domain, and a C-terminal fibronectin-like domain. Several genes appear to be ubiquitously expressed during barley growth and development, while four newly annotated xylanase and xylosidase genes are expressed at extremely high levels, which may be of broader interest for industrial applications where cell wall degradation is necessary.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Collins, Helen M.Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia. (författare)
  • Shirley, Neil J.Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia. (författare)
  • Cuesta-Seijo, Jose A.Carlsberg Res Lab, JC Jacobsens Gade 4, DK-1799 Copenhagen V, Denmark. (författare)
  • Schwerdt, Julian G.Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia. (författare)
  • Phillips, Renee J.Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia. (författare)
  • Finnie, ChristineCarlsberg Res Lab, JC Jacobsens Gade 4, DK-1799 Copenhagen V, Denmark. (författare)
  • Fincher, Geoffrey B.Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia. (författare)
  • Dockter, ChristophCarlsberg Res Lab, JC Jacobsens Gade 4, DK-1799 Copenhagen V, Denmark. (författare)
  • Skadhauge, BirgitteCarlsberg Res Lab, JC Jacobsens Gade 4, DK-1799 Copenhagen V, Denmark. (författare)
  • Bulone, VincentKTH,Glykovetenskap,Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia.;Royal Inst Technol KTH, AlbaNova Univ Ctr, Sch Engn Sci Chem Biotechnol & Hlth, Div Glycosci,Dept Chem, S-10691 Stockholm, Sweden.(Swepub:kth)u13o1auq (författare)
  • Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia.Carlsberg Res Lab, JC Jacobsens Gade 4, DK-1799 Copenhagen V, Denmark. (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Plant Science: Elsevier BV3080168-94521873-2259

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