Sökning: onr:"swepub:oai:DiVA.org:kth-297829" >
The effect of therm...
The effect of thermostabilising mutations on the pressure stability of Trichoderma reesei GH11 xylanase
-
- Li, He (författare)
- Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, PO Box 16100, FI-00076 Aalto, Finland
-
- Murtomäki, Lasse (författare)
- Department of Chemistry, School of Chemical Technology, Aalto University, PO Box 16100, FI-00076 Aalto, Finland
-
- Leisola, Matti (författare)
- Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, PO Box 16100, FI-00076 Aalto, Finland
-
visa fler...
-
- Turunen, Ossi (författare)
- Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, PO Box 16100, FI-00076 Aalto, Finland
-
visa färre...
-
(creator_code:org_t)
- 2012-08-31
- 2012
- Engelska.
-
Ingår i: Protein Engineering, Design & Selection. - : Oxford University Press. - 1741-0134 .- 1741-0126. ; 25:12, s. 821-826
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://academic.oup...
-
https://academic.oup...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- We studied the pressure stability of disulphide bridge mutants of Trichoderma reesei XYNII at 500–5000 bar. The inactivation of XYNII and its mutants was strongest above 4000 bar. The pressure stability correlated with the thermostability order of the XYNII mutants, indicating that the stabilising mutations in protein regions important for thermostability also protect the enzyme at high pressure. In combination with high pressure, a mild heating had already inactivated the wild-type enzyme; the thermostabilising mutations largely counteracted this effect. At a low temperature, the mutations did not have any remarkable pressure stabilisation effect. Thus, thermal inactivation appeared to dominate over pressure inactivation at higher temperatures. Kinetic calculations indicated that pressure compressibility correlated with the thermostability of xylanase mutants.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas