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Sökning: onr:"swepub:oai:DiVA.org:kth-298198" > Quantitative analys...

  • Li, HeDepartment of Chemical and Biological Engineering, ERI, GyeongSang National University, Jinju, Gyeongnam 660-701, 900 Gajwadong, South Korea (författare)

Quantitative analysis of valiolamine through pre-column derivatization with phenylisocyanate using high-performance liquid chromatography with UV detection : Selection of reagent, identification of derivative and optimization of derivatization conditions

  • Artikel/kapitelEngelska2009

Förlag, utgivningsår, omfång ...

  • Elsevier,2009
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:kth-298198
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-298198URI
  • https://doi.org/10.1016/j.jpba.2009.02.020DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • QC 20210630
  • This report describes the improved quantitative determination of valiolamine in a medium for microbial culture using high-performance liquid chromatography with UV detection. Valiolamine aqueous solution was dried, dissolved in dimethyl sulfoxide and derivatization performances of phenylisocyanate (PHI), 1-fluoro-2,4-dinitrobenznene and 1-naphthylisothiocyanate were compared in the presence of triethylamine. The PHI was chosen as the most suitable derivatization reagent and the valiolamine-PHI derivative was identified by thin-layer chromatography and electrospray ionization mass spectrometry. The derivative eluted at 10.5 min on a reverse-phase column using a mobile phase composed of 10% acetonitrile in water containing 0.5 mM sodium octyl sulfate (pH 3.0), at a column flow rate of 1.0 mL/min with UV detection at 240 nm. The optimum conditions for derivatization were a reaction temperature of 30 degrees C, reaction time of 30 min, and PHI concentration higher than 33.6 mM. Calibration curves were linear in the range of 0.99-19.95 microg/mL for the standard solutions and 24.9-99.7 microg/mL for the spiked sample. The proposed method was validated and proven to be selective, accurate and precise and suitable for the quantitative analysis of valiolamine in medium for microbial cultures.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Lee, Jae RanDepartment of Chemical and Biological Engineering, ERI, GyeongSang National University, Jinju, Gyeongnam 660-701, 900 Gajwadong, South Korea (författare)
  • Nguyen, Do Quynh AnhDepartment of Chemical and Biological Engineering, ERI, GyeongSang National University, Jinju, Gyeongnam 660-701, 900 Gajwadong, South Korea (författare)
  • Kim, Sung BaeDepartment of Chemical and Biological Engineering, ERI, GyeongSang National University, Jinju, Gyeongnam 660-701, 900 Gajwadong, South Korea (författare)
  • Seo, Yang-GonDepartment of Chemical and Biological Engineering, ERI, GyeongSang National University, Jinju, Gyeongnam 660-701, 900 Gajwadong, South Korea (författare)
  • Chang, Yong KeunDepartment of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea (författare)
  • Hong, Soon-KwangDivision of Bioscience and Bioinformatics, Myung Ji University, Yongin, Republic of Korea (författare)
  • Moon, Myung HeeDepartment of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea (författare)
  • Chung, In-YoungDepartment of Electronic and Communications Engineering, KwangWoon University, Seoul, Republic of Korea (författare)
  • Kim, Chang-JoonDepartment of Chemical & Biological Engineering and ERI, GyeongSang National University, 900 Gajwadong, Jinju, Gyeongnam 660-701, Republic of Korea (författare)
  • Department of Chemical and Biological Engineering, ERI, GyeongSang National University, Jinju, Gyeongnam 660-701, 900 Gajwadong, South KoreaDepartment of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Journal of Pharmaceutical and Biomedical Analysis: Elsevier49:4, s. 957-9630731-70851873-264X

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