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Functionalized grap...
Functionalized graphene oxide tablets for sample preparation of drugs in biological fluids : Extraction of ritonavir, a HIV protease inhibitor, from human saliva and plasma using LC–MS/MS
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- Zohdijamil, Zeynab (författare)
- Karolinska institutet
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Hashemi, M. (författare)
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Abdel-Rehim, A. (författare)
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- Laxman, Karthik (författare)
- KTH,Material- och nanofysik
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- Uheida, Abdusalam (författare)
- KTH,Material- och nanofysik
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- Dutta, Joydeep (författare)
- KTH,Material- och nanofysik
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- Abdel-Rehim, Mohamed (författare)
- KTH,Material- och nanofysik
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(creator_code:org_t)
- 2021-06
- 2021
- Engelska.
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Ingår i: BMC Biomedical chromotography. - : Wiley. - 0269-3879 .- 1099-0801. ; 35:12
- Relaterad länk:
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https://doi.org/10.1...
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https://onlinelibrar...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- In this work, graphene oxide–based tablets (GO-Tabs) were prepared by applying a thin layer of functionalized GO on a polyethylene substrate. The GO was functionalized with amine groups (–NH2) by poly(ethylene glycol)bis(3-aminopropyl) terminated (GO-NH2-PEG-NH2). The functionalized GO-Tabs were used for the extraction of ritonavir (RTV) in human saliva samples. RTV in plasma and saliva samples was analyzed using LC–MS/MS. Gradient LC system with MS/MS in the positive-ion mode [electrospray ionization (ESI+)] was used. The transitions m/z 721 → 269.0 and m/z 614 → 421 were used for RTV and the internal standard indinavir, respectively. This study determined the human immunodeficiency virus protease inhibitor RTV in human saliva samples using functionalized GO-Tab and LC–MS/MS, and the method was validated. The standard calibration curve for plasma and saliva samples was constructed from 5.0 to 2000 nmol L−1. The limit of detection was 0.1 nmol L−1, and the limit of quantification was 5.0 nmol L−1 in both plasma and saliva matrices. The intra- and inter-assay precision values were found to be between 1.5 and 5.8%, and the accuracy values ranged from 88.0 to 108% utilizing saliva and plasma samples. The extraction recovery was more than 80%, and the presented functionalized GO-Tabs could be reused for more than 10 extractions without deterioration in recovery.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- graphene oxide tablets
- LC–MS/MS
- plasma
- ritonavir
- saliva
- amine
- graphene oxide
- macrogol derivative
- nanocomposite
- poly(ethylene glycol)bis (3 aminopropyl)
- polyethylene
- unclassified drug
- graphite
- Human immunodeficiency virus proteinase inhibitor
- nanomaterial
- blood sampling
- Conference Paper
- drug determination
- drug synthesis
- human
- hydrogen bond
- limit of detection
- limit of quantitation
- liquid chromatography-mass spectrometry
- positive ion electrospray
- saliva analysis
- tablet
- chemistry
- liquid chromatography
- procedures
- reproducibility
- statistical model
- tablet manufacture
- tandem mass spectrometry
- Chromatography
- Liquid
- HIV Protease Inhibitors
- Humans
- Linear Models
- Nanostructures
- Reproducibility of Results
- Tablets
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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