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RecA finds homologo...
RecA finds homologous DNA by reduced dimensionality search
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- Wiktor, Jakub (författare)
- Uppsala universitet,Molekylär systembiologi,Science for Life Laboratory, SciLifeLab
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- Gynnå, Arvid H., 1988- (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylär systembiologi
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- Leroy, Prune (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylär systembiologi
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- Larsson, Jimmy, 1977- (författare)
- Uppsala universitet,Molekylär systembiologi,Science for Life Laboratory, SciLifeLab
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- Coceano, Giovanna (författare)
- KTH,Biofysik,Science for Life Laboratory, SciLifeLab,Department of Applied Physics, Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden
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- Testa, Ilaria (författare)
- KTH,Biofysik,Science for Life Laboratory, SciLifeLab,Department of Applied Physics, Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden
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- Elf, Johan (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylär systembiologi
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(creator_code:org_t)
- 2021-09-01
- 2021
- Engelska.
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Ingår i: Nature. - : Springer Nature. - 0028-0836 .- 1476-4687. ; 597:7876, s. 426-429
- Relaterad länk:
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https://doi.org/10.1...
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https://www.nature.c...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Homologous recombination is essential for the accurate repair of double-stranded DNA breaks (DSBs)1. Initially, the RecBCD complex2 resects the ends of the DSB into 3′ single-stranded DNA on which a RecA filament assembles3. Next, the filament locates the homologous repair template on the sister chromosome4. Here we directly visualize the repair of DSBs in single cells, using high-throughput microfluidics and fluorescence microscopy. We find that, in Escherichia coli, repair of DSBs between segregated sister loci is completed in 15 ± 5 min (mean ± s.d.) with minimal fitness loss. We further show that the search takes less than 9 ± 3 min (mean ± s.d) and is mediated by a thin, highly dynamic RecA filament that stretches throughout the cell. We propose that the architecture of the RecA filament effectively reduces search dimensionality. This model predicts a search time that is consistent with our measurement and is corroborated by the observation that the search time does not depend on the length of the cell or the amount of DNA. Given the abundance of RecA homologues5, we believe this model to be widely conserved across living organisms.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Nyckelord
- cell
- DNA
- fluorescence
- homology
- microscopy
- article
- controlled study
- double strand break repair
- Escherichia coli
- female
- fluorescence microscopy
- microfluidics
- nonhuman
- biological model
- double stranded DNA break
- enzymology
- genetics
- metabolism
- recombination repair
- sequence homology
- time factor
- bacterial DNA
- RecA protein
- single stranded DNA
- DNA Breaks
- Double-Stranded
- DNA
- Bacterial
- DNA
- Single-Stranded
- Models
- Biological
- Rec A Recombinases
- Recombinational DNA Repair
- Sequence Homology
- Nucleic Acid
- Time Factors
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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