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Bacteria Detection ...
Bacteria Detection at a Single-Cell Level through a Cyanotype-Based Photochemical Reaction
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- Dietvorst, Jiri (författare)
- CSIC, Inst Microelect Barcelona IMB CNM, Bellaterra 08193, Barcelona, Spain.;CSIC, Inst Adv Chem Catalonia IQAC, Dept Chem & Biomol Nanotechnol, Nanobiotechnol Diagnost Nb4D, Barcelona 08034, Spain.
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- Ferrer-Vilanova, Amparo (författare)
- CSIC, Inst Microelect Barcelona IMB CNM, Bellaterra 08193, Barcelona, Spain.;Univ Autonoma Barcelona, Dept Quim, Bellaterra 08193, Barcelona, Spain.
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- Iyengar, Sharath Narayana (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Russom, Aman, Prof. 1976- (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Vigues, Nuria (författare)
- Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Barcelona, Spain.
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- Mas, Jordi (författare)
- Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Barcelona, Spain.
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- Vilaplana, Lluisa (författare)
- CSIC, Inst Adv Chem Catalonia IQAC, Dept Chem & Biomol Nanotechnol, Nanobiotechnol Diagnost Nb4D, Barcelona 08034, Spain.
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- Marco, Maria-Pilar (författare)
- CSIC, Inst Adv Chem Catalonia IQAC, Dept Chem & Biomol Nanotechnol, Nanobiotechnol Diagnost Nb4D, Barcelona 08034, Spain.;CIBER Bioingn Biomat & Nanomed CIBER BBN, Barcelona 08034, Spain.
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- Guirado, Gonzalo (författare)
- Univ Autonoma Barcelona, Dept Quim, Bellaterra 08193, Barcelona, Spain.
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- Munoz-Berbel, Xavier (författare)
- CSIC, Inst Microelect Barcelona IMB CNM, Bellaterra 08193, Barcelona, Spain.
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CSIC, Inst Microelect Barcelona IMB CNM, Bellaterra 08193, Barcelona, Spain;CSIC, Inst Adv Chem Catalonia IQAC, Dept Chem & Biomol Nanotechnol, Nanobiotechnol Diagnost Nb4D, Barcelona 08034, Spain. CSIC, Inst Microelect Barcelona IMB CNM, Bellaterra 08193, Barcelona, Spain.;Univ Autonoma Barcelona, Dept Quim, Bellaterra 08193, Barcelona, Spain. (creator_code:org_t)
- 2021-12-21
- 2022
- Engelska.
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 94:2, s. 787-792
- Relaterad länk:
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https://doi.org/10.1...
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https://pubs.acs.org...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The detection of living organisms at very low concentrations is necessary for the early diagnosis of bacterial infections, but it is still challenging as there is a need for signal amplification. Cell culture, nucleic acid amplification, or nano-structure-based signal enhancement are the most common amplification methods, relying on long, tedious, complex, or expensive procedures. Here, we present a cyanotype-based photochemical amplification reaction enabling the detection of low bacterial concentrations up to a single-cell level. Photocatalysis is induced with visible light and requires bacterial metabolism of iron-based compounds to produce Prussian Blue. Bacterial activity is thus detected through the formation of an observable blue precipitate within 3 h of the reaction, which corresponds to the concentration of living organisms. The short time-to-result and simplicity of the reaction are expected to strongly impact the clinical diagnosis of infectious diseases.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
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Dietvorst, Jiri
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Ferrer-Vilanova, ...
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Iyengar, Sharath ...
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Russom, Aman, Pr ...
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Vigues, Nuria
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Mas, Jordi
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Vilaplana, Lluis ...
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Marco, Maria-Pil ...
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Guirado, Gonzalo
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Munoz-Berbel, Xa ...
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Analytisk kemi
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Analytical Chemi ...
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