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An Optimized ChIP-S...
An Optimized ChIP-Seq Protocol to Determine Chromatin Binding of Estrogen Receptor Beta.
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- Indukuri, Rajitha (författare)
- KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden
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- Damdimopoulos, Anastasios (författare)
- Karolinska Institutet
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- Williams, Cecilia, Professor, 1969- (författare)
- Karolinska Institutet,KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden,Cecilia Williams
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(creator_code:org_t)
- 2022-02-04
- 2022
- Engelska.
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Ingår i: Methods in Molecular Biology. - New York, NY : Springer Nature. - 1064-3745 .- 1940-6029. ; 2418, s. 203-221
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Estrogen regulates transcription through two nuclear receptors, ERα and ERβ, in a tissue and cellular-dependent manner. Both the receptors bind estrogen and activate transcription through direct or indirect interactions with DNA. Revealing their interactions with the chromatin is key to understanding their transcriptional activities and their biological functions. Chromatin-immunoprecipitation followed by sequencing (ChIP-Seq) is a powerful technique to map protein-DNA interactions at precise genomic locations. The genome-wide binding of ERα has been extensively studied. Similar studies of ERβ, however, have been more difficult, in part due to a lack of endogenous expression in cell lines and lack of specific antibodies. In this chapter, we provide an optimized stepwise ChIP protocol for a well-validated ERβ antibody, which is applicable for ChIP-Seq analysis of cell lines with exogenous expression of ERβ.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Antibody
- ChIP-Seq
- Chromatin binding
- Estrogen receptor beta
- Immunoprecipitation
- Transcription factor
- Bioteknologi
- Biotechnology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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