Sökning: onr:"swepub:oai:DiVA.org:kth-316289" >
3D Bioprinting of M...
3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures
-
- Khati, Vamakshi (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
- Ramachandraiah, Harisha (författare)
- Biopr AB, S-17165 Solna, Sweden.
-
- Pati, Falguni (författare)
- Indian Inst Technol Hyderabad, Dept Biomed Engn, Kandi 502285, India.
-
visa fler...
-
- Svahn Andersson, Helene (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
- Gaudenzi, Giulia (författare)
- Karolinska Institutet,KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab,Department of Global Public Health, Karolinska Institute, 17165 Solna, Sweden
-
- Russom, Aman, Prof. 1976- (författare)
- Karolinska Institutet,KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab,AIMES—Center for the Advancement of Integrated Medical and Engineering Sciences, Karolinska Institute and KTH Royal Institute of Technology, 11428 Stockholm, Sweden
-
visa färre...
-
(creator_code:org_t)
- 2022-07-13
- 2022
- Engelska.
-
Ingår i: Biosensors. - : MDPI AG. - 2079-6374. ; 12:7
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.3...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Bioprinting is an acclaimed technique that allows the scaling of 3D architectures in an organized pattern but suffers from a scarcity of appropriate bioinks. Decellularized extracellular matrix (dECM) from xenogeneic species has garnered support as a biomaterial to promote tissue-specific regeneration and repair. The prospect of developing dECM-based 3D artificial tissue is impeded by its inherent low mechanical properties. In recent years, 3D bioprinting of dECM-based bioinks modified with additional scaffolds has advanced the development of load-bearing constructs. However, previous attempts using dECM were limited to low-temperature bioprinting, which is not favorable for a longer print duration with cells. Here, we report the development of a multi-material decellularized liver matrix (dLM) bioink reinforced with gelatin and polyethylene glycol to improve rheology, extrudability, and mechanical stability. This shear-thinning bioink facilitated extrusion-based bioprinting at 37 degrees C with HepG2 cells into a 3D grid structure with a further enhancement for long-term applications by enzymatic crosslinking with mushroom tyrosinase. The heavily crosslinked structure showed a 16-fold increase in viscosity (2.73 Pa s(-1)) and a 32-fold increase in storage modulus from the non-crosslinked dLM while retaining high cell viability (85-93%) and liver-specific functions. Our results show that the cytocompatible crosslinking of dLM bioink at physiological temperatures has promising applications for extended 3D-printing procedures.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- decellularized liver matrix bioink
- bioprinting at physiological temperatures
- cytocompatible crosslinking
- robust bioink
- viscoelasticity
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas