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Structural Properti...
Structural Properties Dictating Selective Optotracer Detection of Staphylococcus aureus
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- Butina, Karen (författare)
- KTH,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES,Department of Neuroscience, Karolinska Institutet, Solnavägen 9, Stockholm, 171 77, Sweden,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden; Karolinska Inst, Sweden
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- Lantz, Linda, 1985- (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Choong, Ferdinand X. (författare)
- Karolinska Institutet,KTH,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES,Department of Neuroscience, Karolinska Institutet, Solnavägen 9, Stockholm, 171 77, Sweden,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden; Karolinska Inst, Sweden
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- Tomac, Ana (författare)
- KTH,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES,Department of Neuroscience, Karolinska Institutet, Solnavägen 9, Stockholm, 171 77, Sweden,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden; Karolinska Inst, Sweden
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- Shirani, Hamid, 1967- (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Löffler, Susanne (författare)
- Karolinska Institutet
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- Nilsson, Peter, 1970- (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden
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- Richter-Dahlfors, Agneta (författare)
- Karolinska Institutet,KTH,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES,Department of Neuroscience, Karolinska Institutet, Solnavägen 9, Stockholm, 171 77, Sweden,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden; Karolinska Inst, Sweden
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(creator_code:org_t)
- 2022-04
- 2022
- Engelska.
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Ingår i: ChemBioChem. - : Wiley. - 1439-4227 .- 1439-7633. ; 23:11
- Relaterad länk:
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https://doi.org/10.1...
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Optotracers are conformation-sensitive fluorescent tracer molecules that detect peptide- and carbohydrate-based biopolymers. Their binding to bacterial cell walls allows selective detection and visualisation of Staphylococcus aureus (S. aureus). Here, we investigated the structural properties providing optimal detection of S. aureus. We quantified spectral shifts and fluorescence intensity in mixes of bacteria and optotracers, using automatic peak analysis, cross-correlation, and area-under-curve analysis. We found that the length of the conjugated backbone and the number of charged groups, but not their distribution, are important factors for selective detection of S. aureus. The photophysical properties of optotracers were greatly improved by incorporating a donor-acceptor-donor (D-A-D)-type motif in the conjugated backbone. With significantly reduced background and binding-induced on-switch of fluorescence, these optotracers enabled real-time recordings of S. aureus growth. Collectively, this demonstrates that chemical structure and photophysics are key tunable characteristics in the development of optotracers for selective detection of bacterial species.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- bacterial detection
- fluorescence microscopy
- fluorescence spectroscopy
- optotracers
- real-time monitoring
- adult
- area under the curve
- article
- bacterium detection
- conformation
- cross correlation
- fluorescence intensity
- nonhuman
- quantitative analysis
- spectrofluorometry
- Staphylococcus aureus
- bacterium
- fluorescence resonance energy transfer
- Bacteria
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Butina, Karen
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Lantz, Linda, 19 ...
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Choong, Ferdinan ...
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Tomac, Ana
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Shirani, Hamid, ...
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Löffler, Susanne
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Nilsson, Peter, ...
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Richter-Dahlfors ...
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Analytisk kemi
- Artiklar i publikationen
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ChemBioChem
- Av lärosätet
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Kungliga Tekniska Högskolan
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Linköpings universitet
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Karolinska Institutet