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The cellulose synth...
The cellulose synthase-like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley
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Lou, H. (författare)
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Tucker, M. R. (författare)
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Shirley, N. J. (författare)
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visa fler...
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Lahnstein, J. (författare)
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Yang, X. (författare)
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Ma, C. (författare)
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Schwerdt, J. (författare)
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Fusi, R. (författare)
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Burton, R. A. (författare)
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Band, L. R. (författare)
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Bennett, M. J. (författare)
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- Bulone, Vincent (författare)
- KTH,Glykovetenskap,School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia, 5064 Australia ARC Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia, 5064 Australia Adelaide Glycomics, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia, 5064 Australia
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(creator_code:org_t)
- 2022-05-04
- 2022
- Engelska.
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Ingår i: The Plant Journal. - : Wiley. - 0960-7412 .- 1365-313X. ; 110:6, s. 1681-1699
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The barley cellulose synthase-like F (CslF) genes encode putative cell wall polysaccharide synthases. They are related to the cellulose synthase (CesA) genes involved in cellulose biosynthesis, and the CslD genes that influence root hair development. Although CslD genes are implicated in callose, mannan and cellulose biosynthesis, and are found in both monocots and eudicots, CslF genes are specific to the Poaceae. Recently the barley CslF3 (HvCslF3) gene was shown to be involved in the synthesis of a novel (1,4)-β-linked glucoxylan, but it remains unclear whether this gene contributes to plant growth and development. Here, expression profiling using qRT-PCR and mRNA in situ hybridization revealed that HvCslF3 accumulates in the root elongation zone. Silencing HvCslF3 by RNAi was accompanied by slower root growth, linked with a shorter elongation zone and a significant reduction in root system size. Polymer profiling of the RNAi lines revealed a significant reduction in (1,4)-β-linked glucoxylan levels. Remarkably, the heterologous expression of HvCslF3 in wild-type (Col-0) and root hair-deficient Arabidopsis mutants (csld3 and csld5) complemented the csld5 mutant phenotype, in addition to altering epidermal cell fate. Our results reveal a key role for HvCslF3 during barley root development and suggest that members of the CslD and CslF gene families have similar functions during root growth regulation.
Ämnesord
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Arabidopsis
- barley
- cell wall
- cellulose synthase-like gene
- glucoxylan
- Hordeum vulgare
- Oroot development
- Biochemistry
- Biosynthesis
- Cellulose
- Cytology
- Plants (botany)
- Cell walls
- Cell-wall-polysaccharides
- Cellulose synthase
- Root growth
- Genes
- glucosyltransferase
- polysaccharide
- gene expression regulation
- genetics
- Hordeum
- metabolism
- Gene Expression Regulation
- Plant
- Glucosyltransferases
- Polysaccharides
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Lou, H.
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Tucker, M. R.
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Shirley, N. J.
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Lahnstein, J.
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Yang, X.
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Ma, C.
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visa fler...
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Schwerdt, J.
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Fusi, R.
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Burton, R. A.
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Band, L. R.
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Bennett, M. J.
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Bulone, Vincent
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visa färre...
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