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Sökning: onr:"swepub:oai:DiVA.org:kth-325236" > Multi-marker profil...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004864naa a2200541 4500
001oai:DiVA.org:kth-325236
003SwePub
008230403s2023 | |||||||||||000 ||eng|
009oai:DiVA.org:uu-500017
009oai:prod.swepub.kib.ki.se:152073271
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3252362 URI
0247 a https://doi.org/10.1016/j.bios.2023.1151422 DOI
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5000172 URI
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:1520732712 URI
040 a (SwePub)kthd (SwePub)uud (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Sahu, Siddharth S.u KTH,Tillämpad fysik,KTH Royal Inst Technol, Sch Engn Sci, Dept Appl Phys, S-10691 Stockholm, Sweden.4 aut0 (Swepub:kth)u1oohxvn
2451 0a Multi-marker profiling of extracellular vesicles using streaming current and sequential electrostatic labeling
264 1b Elsevier BV,c 2023
338 a print2 rdacarrier
500 a QC 20230403
520 a High heterogeneity in the membrane protein expression of small extracellular vesicles (sEVs) means that bulk methods relying on antibody-based capture for expression analysis have a drawback that each type of antibody may capture a different sub-population. An improved approach is to capture a representative sEV population, without any bias, and then perform a multiplexed protein expression analysis on this population. However, such a possibility has been largely limited to fluorescence-based methods. Here, we present a novel electrostatic labelling strategy and a microchip-based all-electric method for membrane protein analysis of sEVs. The method allows us to profile multiple surface proteins on the captured sEVs using alternating charge labels. It also permits the comparison of expression levels in different sEV-subtypes. The proof of concept was tested by capturing sEVs both non-specifically (unbiased) as well as via anti-CD9 capture probes (biased), and then profiling the expression levels of various surface proteins using the charge labelled antibodies. The method is the first of its kind, demonstrating an all-electrical and microchip based method that allows for unbiased analysis of sEV membrane protein expression, comparison of expression levels in different sEV subsets, and fractional estimation of different sEV sub-populations. These results were also validated in parallel using a single-sEV fluorescence technique.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
653 a Biosensors
653 a Extracellular vesicles
653 a Streaming current
653 a Electrostatic labels
653 a Microfluidics
7001 a Talebian Gevari, Moeinu Uppsala universitet,Fasta tillståndets elektronik4 aut0 (Swepub:uu)moeta494
7001 a Nagy, Abelu KTH,Proteinvetenskap,KTH Royal Inst Technol, Sch Chem Biotechnol & Hlth CBH, Dept Prot Sci, Stockholm, Sweden.4 aut0 (Swepub:kth)u1kg1ew9
7001 a Gestin, Maxime,d 1990-u KTH,Proteinvetenskap,KTH Royal Inst Technol, Sch Chem Biotechnol & Hlth CBH, Dept Prot Sci, Stockholm, Sweden.4 aut0 (Swepub:kth)u18nn0n1
7001 a Haag, Petrau Karolinska Institutet4 aut
7001 a Lewensohn, Rolfu Karolinska Inst, Dept Oncol Pathol, S-17164 Stockholm, Sweden.;Karolinska Univ Hosp, Thorac Oncol Ctr, Med Unit Head & Neck Lung & Skin Tumors, Theme Canc, S-17164 Solna, Sweden.4 aut
7001 a Viktorsson, Kristinau Karolinska Institutet4 aut
7001 a Eriksson Karlström, Amelieu KTH,Proteinvetenskap,KTH Royal Inst Technol, Sch Chem Biotechnol & Hlth CBH, Dept Prot Sci, Stockholm, Sweden.4 aut0 (Swepub:kth)u10x6l4n
7001 a Dev, Apurbau Uppsala universitet,KTH,Tillämpad fysik,Uppsala Univ, Dept Elect Engn, Div Solid State Elect, S-75121 Uppsala, Sweden.,Fasta tillståndets elektronik,KTH Royal Inst Technol, Sch Engn Sci, Dept Appl Phys, S-10691 Stockholm, Sweden.4 aut0 (Swepub:uu)apude110
7102 a KTHb Tillämpad fysik4 org
7730 t Biosensors & bioelectronicsd : Elsevier BVg 227q 227x 0956-5663x 1873-4235
8564 u https://doi.org/10.1016/j.bios.2023.115142y Fulltext
8564 u https://uu.diva-portal.org/smash/get/diva2:1751773/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-325236
8564 8u https://doi.org/10.1016/j.bios.2023.115142
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-500017
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:152073271

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