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Molecular cryo-elec...
Molecular cryo-electron tomography of vitreous tissue sections : current challenges
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- Norlén, L. (författare)
- Karolinska Institutet
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- Öktem, Ozan (författare)
- KTH,Strategiskt centrum för industriell och tillämpad matematik, CIAM,Matematik (Inst.)
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- Skoglund, U. (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2009-08-20
- 2009
- Engelska.
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Ingår i: Journal of Microscopy. - : Wiley. - 0022-2720 .- 1365-2818. ; 235:3, s. 293-307
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Electron tomography of vitreous tissue sections (tissue TOVIS) allows the study of the three-dimensional structure of molecular complexes in a near-native cellular context. Its usage is, however, limited by an unfortunate combination of noisy and incomplete data, by a technically demanding sample preparation procedure, and by a disposition for specimen degradation during data collection. Here we outline some major challenges as experienced from the application of TOVIS to human skin. We further consider a number of practical measures as well as theoretical approaches for its future development.
Nyckelord
- CEMOVIS
- electron tomography
- regularization
- skin
- tomography of vitreous sections
- TOVIS
- TECHNOLOGY
- TEKNIKVETENSKAP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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