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Biosynthesis of Fur...
Biosynthesis of Furfurylamines in Batch and Continuous Flow by Immobilized Amine Transaminases
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- Heinks, Tobias (författare)
- Bielefeld Univ, Fac Chem Biochem, Univ Str 25, D-33615 Bielefeld, Germany.
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- Merz, Luisa M. (författare)
- KTH,Industriell bioteknologi,Albanova VinnExcellence Center for Protein Technology, ProNova,ViaZym BV, Molengraaffsingel 10, NL-2629JD Delft, Netherlands.
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- Liedtke, Jan (författare)
- Bielefeld Univ, Fac Chem Biochem, Univ Str 25, D-33615 Bielefeld, Germany.
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- Hoehne, Matthias (författare)
- Tech Univ Berlin, Biocatalysis Biol Chem, Muller Breslau Str 10, D-10623 Berlin, Germany.
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- van Langen, Luuk M. (författare)
- ViaZym BV, Molengraaffsingel 10, NL-2629JD Delft, Netherlands.
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- Bornscheuer, Uwe T. (författare)
- Univ Greifswald, Inst Biochem, Dept Biotechnol & Enzyme Catalysis, Felix Hausdorff Str 4, D-17487 Greifswald, Germany.
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- von Mollard, Gabriele Fischer (författare)
- Bielefeld Univ, Fac Chem Biochem, Univ Str 25, D-33615 Bielefeld, Germany.
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- Berglund, Per (författare)
- KTH,Industriell bioteknologi,Albanova VinnExcellence Center for Protein Technology, ProNova
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Bielefeld Univ, Fac Chem Biochem, Univ Str 25, D-33615 Bielefeld, Germany Industriell bioteknologi (creator_code:org_t)
- MDPI AG, 2023
- 2023
- Engelska.
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Ingår i: Catalysts. - : MDPI AG. - 2073-4344. ; 13:5, s. 875-
- Relaterad länk:
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https://doi.org/10.3...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Building blocks with amine functionality are crucial in the chemical industry. Biocatalytic syntheses and chemicals derived from renewable resources are increasingly desired to achieve sustainable production of these amines. As a result, renewable materials such as furfurals, especially furfurylamines like 5-(hydroxymethyl)furfurylamine (HMFA) and 2,5-di(aminomethyl)furan (DAF), are gaining increasing attention. In this study, we identified four different amine transaminases (ATAs) that catalyze the reductive amination of 5-(hydroxymethyl)furfural (HMF) and 2,5-diformylfuran (DFF). We successfully immobilized these ATAs on glutaraldehyde-functionalized amine beads using multiple binding and on amine beads by site-selective binding of the unique Ca-formylglycine within an aldehyde tag. All immobilized ATAs were efficiently reused in five repetitive cycles of reductive amination of HMF with alanine as co-substrate, while the ATA from Silicibacter pomeroyi (ATA-Spo) also exhibited high stability for reuse when isopropylamine was used as an amine donor. Additionally, immobilized ATA-Spo yielded high conversion in the batch syntheses of HMFA and DAF using alanine (87% and 87%, respectively) or isopropylamine (99% and 98%, respectively) as amine donors. We further demonstrated that ATA-Spo was effective for the reductive amination of HMF with alanine or isopropylamine in continuous-flow catalysis with high conversion up to 12 days (48% and 41%, respectively).
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- amine transaminase
- biocatalysis
- DFF
- flow synthesis
- HMF
- immobilization
- isopropylamine
- reuse stability
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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