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Single-Step Quantif...
Single-Step Quantification of Specific Nucleic Acid Sequences in Microfluidics Using a Multilabeled Hybrid DNA Duplex
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- Pinto, Ines F. (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Soares, Ruben R. G. (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi
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- Russom, Aman, Prof. 1976- (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi,Division of Nanobiotechnology, Department of Protein Science, Science for Life Laboratory, Solna, Sweden; AIMES - Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet and KTH Royal Institute of Technology, Stockholm, Sweden
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(creator_code:org_t)
- Chemical and Biological Microsystems Society, 2021
- 2021
- Engelska.
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Ingår i: MicroTAS 2021. - : Chemical and Biological Microsystems Society. ; , s. 757-758
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- The rapid and specific detection of nucleic acid sequences is highly demanded for several applications including pathogen diagnostics, quality control of biopharmaceutical products and forensics. Nucleic acid amplification methods based on mixtures of primers and polymerase enzymes such as polymerase chain reaction (PCR) and other isothermal methods are typically the standard approach. Here, using SARS-CoV-2 ORF1ab sequence as a model, we report the development of a simple enzyme-free and single-step competitive hybridization method allowing the specific detection of any type of nucleic acid sequence (ss/dsDNA or RNA) within 15 min with 89% sequence homology and sensitivity in the pM-range.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Beads
- Competitive
- Corona Virus
- Hybridization
Publikations- och innehållstyp
- ref (ämneskategori)
- kon (ämneskategori)