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Field testing of tr...
Field testing of transgenic aspen from large greenhouse screening identifies unexpected winners
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- Donev, Evgeniy N. (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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- Derba-Maceluch, Marta (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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- Yassin, Zakiya (författare)
- RISE,Bygg och fastighet,Enhet Produktionssystem och Material, RISE Research Institutes of Sweden, Växjö, Sweden
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- Gandla, Madhavi Latha (författare)
- Umeå universitet,Kemiska institutionen,Department of Chemistry, Umeå University, Umeå, Sweden
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- Sivan, Pramod, 1984- (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,KTH,Glykovetenskap,Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå, Sweden; Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology, AlbaNova University Centre, Stockholm, Sweden,SLU Swedish University of Agricultural Sciences, Sweden; KTH Royal Institute of Technology, Sweden,Institutionen för skoglig genetik och växtfysiologi,Royal Institute of Technology (KTH)
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- Heinonen, Emilia (författare)
- KTH,Wallenberg Wood Science Center,Glykovetenskap,Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology, AlbaNova University Centre, Stockholm, Sweden; Wallenberg Wood Science Centre (WWSC), KTH Royal Institute of Technology, Stockholm, Sweden
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- Kumar, Vikash (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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- Scheepers, Gerhard (författare)
- RISE,Bygg och fastighet,Enhet Produktionssystem och Material, RISE Research Institutes of Sweden, Växjö, Sweden
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- Vilaplana, Francisco, 1979- (författare)
- KTH,Glykovetenskap,Wallenberg Wood Science Center,Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology, AlbaNova University Centre, Stockholm, Sweden; Wallenberg Wood Science Centre (WWSC), KTH Royal Institute of Technology, Stockholm, Sweden
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- Johansson, Ulf (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Enheten för skoglig fältforskning,Unit for Field-based Forest Research
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- Hertzberg, Magnus (författare)
- SweTree Technologies AB, Umeå, Sweden
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- Sundberg, Björn (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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- Winestrand, Sandra (författare)
- Umeå universitet,Kemiska institutionen,Department of Chemistry, Umeå University, Umeå, Sweden
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- Hörnberg, Andreas (författare)
- RISE,Bioraffinaderi och energi,RISE Processum AB, Örnsköldsvik, Sweden
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- Alriksson, Björn (författare)
- RISE,Bioraffinaderi och energi,RISE Processum AB, Örnsköldsvik, Sweden
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- Jönsson, Leif J. (författare)
- Umeå universitet,Kemiska institutionen,Department of Chemistry, Umeå University, Umeå, Sweden
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- Mellerowicz, Ewa (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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(creator_code:org_t)
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- 2023-02-03
- 2023
- Engelska.
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Ingår i: Plant Biotechnology Journal. - : Wiley. - 1467-7644 .- 1467-7652. ; 21:5, s. 1005-1021
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://pub.epsilon.... (primary) (Raw object) (free)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://urn.kb.se/re...
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https://res.slu.se/i...
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Abstract
Ämnesord
Stäng
- Trees constitute promising renewable feedstocks for biorefinery using biochemical conversion, but their recalcitrance restricts their attractiveness for the industry. To obtain trees with reduced recalcitrance, large-scale genetic engineering experiments were performed in hybrid aspen blindly targeting genes expressed during wood formation and 32 lines representing seven constructs were selected for characterization in the field. Here we report phenotypes of five-year old trees considering 49 traits related to growth and wood properties. The best performing construct considering growth and glucose yield in saccharification with acid pretreatment had suppressed expression of the gene encoding an uncharacterized 2-oxoglutarate-dependent dioxygenase (2OGD). It showed minor changes in wood chemistry but increased nanoporosity and glucose conversion. Suppressed levels of SUCROSE SYNTHASE, (SuSy), CINNAMATE 4-HYDROXYLASE (C4H) and increased levels of GTPase activating protein for ADP-ribosylation factor ZAC led to significant growth reductions and anatomical abnormalities. However, C4H and SuSy constructs greatly improved glucose yields in saccharification without and with pretreatment, respectively. Traits associated with high glucose yields were different for saccharification with and without pretreatment. While carbohydrates, phenolics and tension wood contents positively impacted the yields without pretreatment and growth, lignin content and S/G ratio were negative factors, the yields with pretreatment positively correlated with S lignin and negatively with carbohydrate contents. The genotypes with high glucose yields had increased nanoporosity and mGlcA/Xyl ratio, and some had shorter polymers extractable with subcritical water compared to wild-type. The pilot-scale industrial-like pretreatment of best-performing 2OGD construct confirmed its superior sugar yields, supporting our strategy.
Ämnesord
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Miljöbioteknik -- Annan miljöbioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Environmental Biotechnology -- Other Environmental Biotechnology (hsv//eng)
Nyckelord
- enzymatic saccharification
- field trial
- secondary cell wall
- SilviScan
- subcritical water extraction
- transgenic Populus
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Donev, Evgeniy N ...
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Derba-Maceluch, ...
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Yassin, Zakiya
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Gandla, Madhavi ...
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Sivan, Pramod, 1 ...
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Heinonen, Emilia
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visa fler...
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Kumar, Vikash
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Scheepers, Gerha ...
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Vilaplana, Franc ...
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Johansson, Ulf
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Hertzberg, Magnu ...
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Sundberg, Björn
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Winestrand, Sand ...
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Hörnberg, Andrea ...
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Alriksson, Björn
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Jönsson, Leif J.
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Mellerowicz, Ewa
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visa färre...
- Om ämnet
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- LANTBRUKSVETENSKAPER
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LANTBRUKSVETENSK ...
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och Bioteknologi med ...
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och Växtbioteknologi
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Miljöbioteknik
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och Annan miljöbiote ...
- Artiklar i publikationen
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Plant Biotechnol ...
- Av lärosätet
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Kungliga Tekniska Högskolan
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Umeå universitet
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RISE
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Sveriges Lantbruksuniversitet