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Multilayer DNA Orig...
Multilayer DNA Origami with Terminal Interfaces That Are Flat and Wide-Area
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- Kilwing, Luzia (författare)
- Ludwig Maximilians Univ Munchen, Fac Phys, D-80539 Munich, Germany.;Ludwig Maximilians Univ Munchen, Ctr Nanosci CeNS, D-80539 Munich, Germany.;Harvard Med Sch, Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA.;Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA.
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- Lill, Pascal (författare)
- Harvard Med Sch, Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA.;Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA.
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- Nathwani, Bhavik (författare)
- Harvard Med Sch, Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA.;Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA.
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- Guerra, Richard (författare)
- Harvard Med Sch, Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA.;Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA.
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- Benson, Erik (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Genteknologi,Univ Oxford, Dept Phys, Clarendon Lab, Oxford OX1 3PU, England.;Univ Oxford, Kavli Inst Nanosci Discovery, Oxford OX1 3PU, England
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- Liedl, Tim (författare)
- Ludwig Maximilians Univ Munchen, Fac Phys, D-80539 Munich, Germany.;Ludwig Maximilians Univ Munchen, Ctr Nanosci CeNS, D-80539 Munich, Germany.
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- Shih, William M. (författare)
- Harvard Med Sch, Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA.;Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA.
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Ludwig Maximilians Univ Munchen, Fac Phys, D-80539 Munich, Germany;Ludwig Maximilians Univ Munchen, Ctr Nanosci CeNS, D-80539 Munich, Germany.;Harvard Med Sch, Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA.;Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA. Harvard Med Sch, Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA.;Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA. (creator_code:org_t)
- American Chemical Society (ACS), 2023
- 2023
- Engelska.
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Ingår i: ACS Nano. - : American Chemical Society (ACS). - 1936-0851 .- 1936-086X. ; 18:1, s. 885-893
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- DNA origami is a popular nanofabrication strategy that employs self-assembly of a long single scaffold strand, typically less than 10 kilobases in length, with hundreds of shorter staple strands into a desired shape. In particular, origami arranged as a single-layer rectangle has proven popular as flat pegboards that can display functionalities at staple-strand breakpoints, off the sides of the constituent double helices, with a similar to 5.3 nm rhombic-lattice spacing. For applications that demand tighter spacing, functionalities can be displayed instead on the termini of helices of multilayer DNA origami. However, pegboards with the greatest addressable surface area are often found to be the most versatile. Given the practical limitations of the length of the scaffold that can be easily realized, designs that minimize the length of each helix would have advantages for maximizing the number of helices and therefore the number of addressable pixels on each terminal surface. Here we present an architecture for multilayer DNA origami displaying flush terminal interfaces from over 200 helices that each are only 5.3 turns in length. We characterize an example using cryo-EM imaging paired with single-particle analysis for further analysis of the global structure.
Ämnesord
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Nyckelord
- DNA origami
- DNA nanotechnology
- cryo-EM
- synthetic biology
- nucleic acids
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- ref (ämneskategori)
- art (ämneskategori)
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