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Experimental valida...
Experimental validation of numerical point spread function calculation including aberration estimation
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- Miora, Ratsimandresy Holinirina Dina (författare)
- Physics Department, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa, Private Bag X1; Institute of Physical Chemistry and Abbe Center of Photonics Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany, Helmholtzweg 4
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- Senftleben, Maximilian (författare)
- KTH,Biofysik,Science for Life Laboratory, SciLifeLab
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- Abrahamsson, Sara (författare)
- Baskin School of Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA, 1156 High Street
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- Rohwer, Erich (författare)
- Physics Department, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa, Private Bag X1
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- Heintzmann, Rainer (författare)
- Institute of Physical Chemistry and Abbe Center of Photonics Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany, Helmholtzweg 4; Leibniz Institute of Photonic Technology, Albert-Einstein Str. 9, 07745 Jena, Germany, Albert-Einstein Str. 9
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- Bosman, Gurthwin (författare)
- Physics Department, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa, Private Bag X1; National Institute for Theoretical and Computational Sciences (NITheCS), Stellenbosch, South Africa
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(creator_code:org_t)
- Optica Publishing Group, 2024
- 2024
- Engelska.
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Ingår i: Optics Express. - : Optica Publishing Group. - 1094-4087. ; 32:12, s. 21887-21908
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Image reconstruction in fluorescence microscopy is highly sensitive to the accuracy of the impulse response, defined as the point spread function (PSF), of the optical system under which the image to reconstruct was acquired. In our previous work, we developed a MATLAB toolbox for accurately calculating realistic vector Fourier-based PSF accounting for any type of aberrations [arXiv, arXiv:2301.13515 (2023)10.48550/arXiv.2301.13515]. In this work, we present a fundamental experimental validation of these numerical methods. The simulated results are found to fit experimental data under different image acquisition conditions at an accuracy higher than 0.97 in normalized cross-correlation. These methods enable a relative contrast of up to 95%.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk bildbehandling (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Image Processing (hsv//eng)
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