Steady-state fluorescence anisotropy studies of molecularly imprinted polymer sensors

QC 20120224
Molecularly imprinted polymers (MIPs) are used as recognition elements in biochemical sensors. In a fluorescence-based MIP sensor system, it can be difficult to distinguish the analyte fluorescence from the fluorescence of the polymer itself. We studied steady-state fluorescence anisotropy of anthracene imprinted in a polymer (polyurethane) matrix. Vertically polarized excitation light was incident on MIP films coated on silicon wafers; vertically and horizontally polarized emission was measured. We compared the fluorescence anisotropy of MIPs with imprinted molecules, MIPs with the imprinted molecules extracted, MIPs with rebound molecules, and nonimprinted control polymers. It is shown that differences in fluorescence anisotropy between the polymers and imprinted fluorescent molecules may provide a means to discriminate the fluorescence of analyte from that of the background polymer.

Ingår i:MOLECULARLY IMPRINTED MATERIALS-2003: Materials Research Society, s. 35-391558997253

Av författaren/redakt...: Chen, Y. C.; Wang, Z. M.; Yan, Mingdi; Prahl, S. A.

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